IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week13/Chemical and Light

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(09/30 PCR)
(Amy's mutants)
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=Amy's mutants=
=Amy's mutants=
[[Image:2008-09-30_amymut.jpg]]
[[Image:2008-09-30_amymut.jpg]]
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=RE digests 10/01=
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Numbers refer to the master plate from 09/30 colony PCR. We digested with EcoRI, SpeI, and XmnI.
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*P38+130 #20
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*P39+130 #31
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*P39+130 #39
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The expected band sizes are 2.2 kb (part), 1.6 kb and 0.4 kb (both vector backbone).
=Ligations/transformations 10/03 =
=Ligations/transformations 10/03 =

Revision as of 10:26, 4 October 2008

Contents

Colony PCR 09/30

Should grow only on carb

  • 1-30: 20, 23, 25 (picked all three) --> P38+130
  • 31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60) --> P39+130

Should grow only on kan

  • 61-90: none --> P116+130
  • 91-120: none --> P117+130
  • 121-150: none --> P108+130

--Upload picture of colony PCR gel--

Picked colony 20 (P38+130) and 31, 39, 60 (P39+130) and grew them up in liquid cultures (LB Amp).

Amy's mutants

Image:2008-09-30_amymut.jpg

RE digests 10/01

Numbers refer to the master plate from 09/30 colony PCR. We digested with EcoRI, SpeI, and XmnI.

  • P38+130 #20
  • P39+130 #31
  • P39+130 #39

The expected band sizes are 2.2 kb (part), 1.6 kb and 0.4 kb (both vector backbone).

Ligations/transformations 10/03

We ligated the following plasmids. The ligations were transformed into XL1-Blue.

  • 108>131
  • 38+51>131
  • 122>131
  • 38+130>101
  • 130>133
  • 130>134

Shewanella

We ethanol precipitated the above ligations in preparation for electroporation of Shewanella.

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