IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week13/Chemical and Light

09/30 PCR

Should grow only on carb

• 1-30: 20, 23, 25 (picked all three)
• 31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60)

Should grow only on kan

• 61-90: none
• 91-120: none
• 121-150: none

--Upload picture of colony PCR gel--

Picked colony 20 (P38+130) and 31, 39, 60 (P39+130) and grew them up in liquid cultures (LB Amp).

Amy's mutants

Ligations/transformations 10/03

We ligated the following plasmids. The ligations were transformed into XL1-Blue.

• 108>131
• 38+51>131
• 122>131
• 38+130>101
• 130>133
• 130>134

Shewanella

We ethanol precipitated the above ligations in preparation for electroporation of Shewanella.