IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week14/Chemical and Light

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(Colony PCR 10/11)
(Colony PCR 10/11)
Line 22: Line 22:
Lanes 1-2: P131XS + P116SP
Lanes 1-2: P131XS + P116SP
-
Lane 3-4: P131XP + P108SP (picked 3, 4)
+
Lane 3-4: P147 (picked 3)
-
Lanes 5-12: P140ES (picked 5, 6)
+
Lanes 5-12: 147 (picked 6)
[[Image:10-11_Colony_PCR_13-24.jpg‎]]
[[Image:10-11_Colony_PCR_13-24.jpg‎]]
-
Lanes 13-14: P140ES
+
Lanes 13-14: P147
-
Lanes 15-24: P142 (picked 17, 23)
+
Lanes 15-24: P146 (picked 17, 23)
[[Image:10-11_Colony_PCR_25-36.jpg‎]]
[[Image:10-11_Colony_PCR_25-36.jpg‎]]
-
Lanes 25-26: P142
+
Lanes 25-26: P146
-
 
+
 +
Lanes 27-36: P144 (picked 30, 35)
[[Image:10-11_Colony_PCR_37-48.jpg‎]]
[[Image:10-11_Colony_PCR_37-48.jpg‎]]
 +
 +
Lanes 37-39: P144
 +
 +
Lanes 40-48: P145 (picked 41, 46)

Revision as of 22:40, 11 October 2008

Mystery Colony PCR 10/08

Colony PCR of putative fungal colonies using primers BBpfx and mtrB500.

Image:10-08_Mystery_PCR_MXHTA.jpg

Lane 1: 1 kb ladder

Lanes 2-6: P136 plate (500 bp)

Lane 7: P140 plate (1.9 kb)

Lanes 8-12: P122>131 plate (1.3 kb)

Colony PCR 10/11

Unadulterated gel

Image:10-11_Colony_PCR_MXHTA.jpg


Image:10-11_Colony_PCR_1-12.jpg‎

Lanes 1-2: P131XS + P116SP

Lane 3-4: P147 (picked 3)

Lanes 5-12: 147 (picked 6)

Image:10-11_Colony_PCR_13-24.jpg‎

Lanes 13-14: P147

Lanes 15-24: P146 (picked 17, 23)

Image:10-11_Colony_PCR_25-36.jpg‎

Lanes 25-26: P146

Lanes 27-36: P144 (picked 30, 35)

Image:10-11_Colony_PCR_37-48.jpg‎

Lanes 37-39: P144

Lanes 40-48: P145 (picked 41, 46)

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