IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week3/Widgetry: Difference between revisions
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=Monday: June 7, 2008= | =Monday: June 7, 2008= | ||
Constructed two new MFCs | |||
grew mtrA, mtrB, wt, and E. coli in LB+lactate liquid cultures | |||
Ran negative control with E. coli in LML media | |||
=Tuesday: June 8, 2008= | =Tuesday: June 8, 2008= | ||
Revision as of 13:08, 9 July 2008
Goals for this week
- Make Orianna's growth media
- construct more chambers to run parallel tests
- set up Keithley for multiple tests
- grow mtrA, mtrB,wt, E. coli in liquid cultures
- negative control E. coli in LB/lactate
- standardize tests (OD, bubbling, etc)
Monday: June 7, 2008
Constructed two new MFCs
grew mtrA, mtrB, wt, and E. coli in LB+lactate liquid cultures
Ran negative control with E. coli in LML media
Tuesday: June 8, 2008
Made Orianna's growth media
Grew wt Shewie, mtrA, mtrB, and mtrC/omcA in Orianna's growth media
-overnight cultures
OD readings from liquid cultures grown in LB + lactate growth media
wt: 2.489
mtrB: 1.711
