IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week5/Chemical and Light: Difference between revisions

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[[Image:7-22_gel_1_MXHTA.jpg]]
[[Image:7-22_gel_1_MXHTA.jpg]]
Lane 1: 1 kb plus ladder
Lane 2: CDF PCR cut XP (~900)
Lane 3: CDF PCR uncut (~900)
Lane 4: P1A cut XP (2750)
Lane 5: P1A uncut (3669)
Lane 6: P17 cut EX (5327)
Lane 7: P17 uncut (5327)
Lane 8: P48 cut XP (769)
Lane 9: P48 uncut (3477)


'''Gel 2'''  
'''Gel 2'''  


[[Image:7-22_gel_2_MXHTA.jpg]]
[[Image:7-22_gel_2_MXHTA.jpg]]
Lane 1: 1 kb plus ladder
Lane 2: P49B cut XP (943)
Lane 3: P49B uncut (3651)
Lane 4: P51 cut EX (5795)
Lane 5: P51 uncut (5795)
Lane 6: P52 cut EX (5412)
Lane 7: P52 uncut (5412)
Lane 8: P63 cut EX (3284)
Lane 9: P63 uncut (3284)


'''Gel 3'''
'''Gel 3'''


[[Image:7-22_gel_3_MXHTA.jpg]]
[[Image:7-22_gel_3_MXHTA.jpg]]
Lane 1: 1 kb plus ladder
Lane 2: P97A cut EX (2091)
Lane 3: P97A uncut (2091)
Lane 4: P97B cut EX (2091)
Lane 5: P97B uncut (2091)

Revision as of 11:09, 22 July 2008

Testing Thermoinducible Lac System

Restreaked Plates of P92-3 in S1 7/21

Restreaked P93 (1:3), P92a, and P93b--all successfully transformed in S1--to detect YFP at 30 and 40 degrees.

Sequencing Parts, Plasmids, Etc.

Primers for Sequencing Remy's Plasmids and Duet Vectors 7/21

Making Thermoinducible cI Lambda System

Primers for Taking Out cI857 from PGW7 7/21

Housekeeping

Making Competent Cells 7/22

PCR

mtrB

7/22: new gradient + R primer

Rx mix (split into 12 samples): 180μL PCR supermix, 4μL mtrB-ApaLI-F primer (20μM), 4μL mtrB-KpnI-R-new (20μM), pipet tip touch of mtrB BB from gel purification, 12μL water Rx: 5min @ 94°C → 35x[45s @ 94°C → 45s @ {52-58 gradient}°C → 2m38s @72°C] → 5min @ 72°C → ∞ @ 4°C

RE digests 07/22

Gel 1

Lane 1: 1 kb plus ladder

Lane 2: CDF PCR cut XP (~900)

Lane 3: CDF PCR uncut (~900)

Lane 4: P1A cut XP (2750)

Lane 5: P1A uncut (3669)

Lane 6: P17 cut EX (5327)

Lane 7: P17 uncut (5327)

Lane 8: P48 cut XP (769)

Lane 9: P48 uncut (3477)

Gel 2

Lane 1: 1 kb plus ladder

Lane 2: P49B cut XP (943)

Lane 3: P49B uncut (3651)

Lane 4: P51 cut EX (5795)

Lane 5: P51 uncut (5795)

Lane 6: P52 cut EX (5412)

Lane 7: P52 uncut (5412)

Lane 8: P63 cut EX (3284)

Lane 9: P63 uncut (3284)

Gel 3

Lane 1: 1 kb plus ladder

Lane 2: P97A cut EX (2091)

Lane 3: P97A uncut (2091)

Lane 4: P97B cut EX (2091)

Lane 5: P97B uncut (2091)

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