IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week6/Chemical and Light: Difference between revisions
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(New page: =Sequencing Parts= =Msking Thermoinducible cI System = ==Digestion of Term + pLambda GFP System and cI857 7/28== {| {{table}} | align="center" style="background:#f0f0f0;"|'''''' | align="c...) |
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Revision as of 19:11, 28 July 2008
Sequencing Parts
Msking Thermoinducible cI System
Digestion of Term + pLambda GFP System and cI857 7/28
| ' | P104a | cI857 RBS | cI857 BIG |
| DNA | 15 uL | 13 uL | 20 uL |
| 10x Buffer | 2.5 uL | 2.5 uL | 2.5 uL |
| 100x BSA | 0.25 uL | 0.25 uL | 0.25 uL |
| Restriction Enzyme 1 | 1 uL EcoRI | 1 uL EcoRI | 1 uL EcoRI |
| Restriction Enzyme 2 | 1 uL XbaI | 1 uL SpeI | 1 uL SpeI |
| Water | 5uL | 7uL | 0uL |
| Total | 25uL | 25uL | 25uL |
