IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week7/Chemical and Light: Difference between revisions
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Lane 15: ompR (~750) | Lane 15: ompR (~750) | ||
=TOPO cloning= | =TOPO cloning 08/04= | ||
==P1, mtrB== | ==P1, mtrB== | ||
These had a few white colonies. Colony PCR, however, indicated that the correct PCR product was not incorporated. | These had a few white colonies. Colony PCR, however, indicated that the correct PCR product was not incorporated. | ||
Revision as of 19:45, 4 August 2008
PCRs 08/04/08
Lane 1: 1 kb plus ladder
Lane 2: cph-EnvZ 50 °C annealing temperature (~2300)
Lane 3: cph-EnvZ 50 °C annealing temperature (~2300)
Lane 4: cph-EnvZ 55 °C annealing temperature (~2300)
Lane 5: cph-EnvZ 55 °C annealing temperature (~2300)
Lane 6: P1 (~3000)
Lane 7: P1 (~3000)
Lane 8: P3 (~3000)
Lane 9: P3 (~3000)
Lane 10: mtrB (~2100)
Lane 11: mtrB (~2100)
Lane 12: HO (~1400)
Lane 13: HO (~1400)
Lane 14: ompR (~750)
Lane 15: ompR (~750)
TOPO cloning 08/04
P1, mtrB
These had a few white colonies. Colony PCR, however, indicated that the correct PCR product was not incorporated.
ompR
All colonies were blue.
HO-pcyA
No colonies.
