IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week7/Chemical and Light

PCRs 08/04/08

Lane 1: 1 kb plus ladder

Lane 2: cph-EnvZ 50 °C annealing temperature (~2300)

Lane 3: cph-EnvZ 50 °C annealing temperature (~2300)

Lane 4: cph-EnvZ 55 °C annealing temperature (~2300)

Lane 5: cph-EnvZ 55 °C annealing temperature (~2300)

Lane 6: P1 (~3000)

Lane 7: P1 (~3000)

Lane 8: P3 (~3000)

Lane 9: P3 (~3000)

Lane 10: mtrB (~2100)

Lane 11: mtrB (~2100)

Lane 12: HO (~1400)

Lane 13: HO (~1400)

Lane 14: ompR (~750)

Lane 15: ompR (~750)

TOPO cloning 08/04

P1, mtrB

These had a few white colonies. Colony PCR, however, indicated that the correct PCR product was not incorporated.

ompR

All colonies were blue.

HO-pcyA

No colonies.

Colony PCR of mtrB samples 08/04

Gel 1

Lane 1: 1 kb plus ladder

Lane 2: P98 (~2100)

Lane 3: P98 (~2100)

Lane 4: P98 (~2100)

Lane 5: P98 (~2100)

Lane 6: P98 (~2100)

Lane 7: P98 (~2100)

Lane 8: P98 (~2100)

Lane 9: P98 (~2100)

Lane 10: P98 (~2100)

Lane 11: P98 (~2100)

Lane 12: P98 (~2100)

Gel 2

Lane 1: 1 kb plus ladder

Lane 2: P98 (~2100)

Lane 3: P98 (~2100)

Lane 4: P98 (~2100)

Lane 5: P98 (~2100)

Lane 6: P98 (~2100)

Lane 7: P98 (~2100)

Lane 8: P98 (~2100)

Lane 9: P98 (~2100)

Lane 10: P98 (~2100)

Lane 11: P98 (~2100)

Lane 12: P98 (~2100)

Gel 3

Lane 1: 1 kb plus ladder

Lane 2: P98+63 (~2100)

Lane 3: P98+63 (~2100)

Lane 4: P98+63 (~2100)

Lane 5: P98+63 (~2100)

Lane 6: P98+63 (~2100)

Lane 7: P98+63 (~2100)

Lane 8: P98+63 (~2100)

Lane 9: P98+63 (~2100)

Lane 10: P98+63 (~2100)

Lane 11: P98+63 (~2100)

Lane 12: P98+63 (~2100)

Gel 4

Lane 1: 1 kb plus ladder

Lane 2: P98+63 (~2100)

Lane 3: P98+63 (~2100)

Lane 4: P98+63 (~2100)

Lane 5: P98+63 (~2100)

Lane 6: P98+63 (~2100)

Lane 7: P98+63 (~2100)

Lane 8: P98+63 (~2100)

Lane 9: P98+63 (~2100)

Lane 10: P98+63 (~2100)

Lane 11: P98+63 (~2100)

Lane 12: P98+63 (~2100)

Gel 5

Lane 1: 1 kb plus ladder

Lane 2: P98+63 (~2100)

Lane 3: P98+63 (~2100)

Lane 4: P98 (~2100)

Lane 5: P98 (~2100)