IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week7/Chemical and Light: Difference between revisions
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| XL10-Gold P75a + P63b (6:2)||Kan||0|| | | XL10-Gold P75a + P63b (6:2)||Kan||0|| | ||
|- | |- | ||
| XL10-Gold P75b + P63b (6:2)||Kan||1|| | | XL10-Gold P75b + P63b (6:2)||Kan||1||fluor | ||
|- | |- | ||
| XL10-Gold P75a + P63b (7:1)||Kan|| | | XL10-Gold P75a + P63b (7:1)||Kan||1||no fluor | ||
|- | |- | ||
| XL10-Gold P75b + P63b (7:1)||Kan||2|| | | XL10-Gold P75b + P63b (7:1)||Kan||2||both fluor | ||
|- | |- | ||
| XL10-Gold Dephos P75a ||Kan||1|| | | XL10-Gold Dephos P75a ||Kan||1|| | ||
Revision as of 15:14, 5 August 2008
PCRs 08/04/08
Lane 1: 1 kb plus ladder
Lane 2: cph-EnvZ 50 °C annealing temperature (~2300)
Lane 3: cph-EnvZ 50 °C annealing temperature (~2300)
Lane 4: cph-EnvZ 55 °C annealing temperature (~2300)
Lane 5: cph-EnvZ 55 °C annealing temperature (~2300)
Lane 6: P1 (~3000)
Lane 7: P1 (~3000)
Lane 8: P3 (~3000)
Lane 9: P3 (~3000)
Lane 10: mtrB (~2100)
Lane 11: mtrB (~2100)
Lane 12: HO (~1400)
Lane 13: HO (~1400)
Lane 14: ompR (~750)
Lane 15: ompR (~750)
TOPO cloning 08/04
P1, mtrB
These had a few white colonies. Colony PCR, however, indicated that the correct PCR product was not incorporated.
ompR
All colonies were blue.
HO-pcyA
No colonies.
Colony PCR of mtrB samples 08/04
Gel 1
Lane 1: 1 kb plus ladder
Lane 2: P98 (~2100)
Lane 3: P98 (~2100)
Lane 4: P98 (~2100)
Lane 5: P98 (~2100)
Lane 6: P98 (~2100)
Lane 7: P98 (~2100)
Lane 8: P98 (~2100)
Lane 9: P98 (~2100)
Lane 10: P98 (~2100)
Lane 11: P98 (~2100)
Lane 12: P98 (~2100)
Gel 2
Lane 1: 1 kb plus ladder
Lane 2: P98 (~2100)
Lane 3: P98 (~2100)
Lane 4: P98 (~2100)
Lane 5: P98 (~2100)
Lane 6: P98 (~2100)
Lane 7: P98 (~2100)
Lane 8: P98 (~2100)
Lane 9: P98 (~2100)
Lane 10: P98 (~2100)
Lane 11: P98 (~2100)
Lane 12: P98 (~2100)
Gel 3
Lane 1: 1 kb plus ladder
Lane 2: P98+63 (~2100)
Lane 3: P98+63 (~2100)
Lane 4: P98+63 (~2100)
Lane 5: P98+63 (~2100)
Lane 6: P98+63 (~2100)
Lane 7: P98+63 (~2100)
Lane 8: P98+63 (~2100)
Lane 9: P98+63 (~2100)
Lane 10: P98+63 (~2100)
Lane 11: P98+63 (~2100)
Lane 12: P98+63 (~2100)
Gel 4
Lane 1: 1 kb plus ladder
Lane 2: P98+63 (~2100)
Lane 3: P98+63 (~2100)
Lane 4: P98+63 (~2100)
Lane 5: P98+63 (~2100)
Lane 6: P98+63 (~2100)
Lane 7: P98+63 (~2100)
Lane 8: P98+63 (~2100)
Lane 9: P98+63 (~2100)
Lane 10: P98+63 (~2100)
Lane 11: P98+63 (~2100)
Lane 12: P98+63 (~2100)
Gel 5
Lane 1: 1 kb plus ladder
Lane 2: P98+63 (~2100)
Lane 3: P98+63 (~2100)
Lane 4: P98 (~2100)
Lane 5: P98 (~2100)
Transforming in XL10-Gold cells
| Plate | Marker | # Colonies | Description |
| XL10-Gold P75a + P63b (1:1) | Kan | 2 | No fluor |
| XL10-Gold P75b + P63b (1:1) | Kan | 6 | 1 fluor |
| XL10-Gold P75a + P63b (6:2) | Kan | 0 | |
| XL10-Gold P75b + P63b (6:2) | Kan | 1 | fluor |
| XL10-Gold P75a + P63b (7:1) | Kan | 1 | no fluor |
| XL10-Gold P75b + P63b (7:1) | Kan | 2 | both fluor |
| XL10-Gold Dephos P75a | Kan | 1 | |
| XL10-Gold Dephos p75b | Kan | 0 | |
| XL10-Gold P3 + (P39+p51) 2/6 | Kan | ||
| XL10-Gold P3 Topo w/ XGAL | Amp | ||
| XL10-Gold P1 Topo w/ XGAL | Amp | ||
| XL10-Gold mtrB Topo w/ XGAL | Amp | ||
| XL10-Gold pUC18 | Amp | 100s | White, very small |
| XL10-Gold negative control | Amp | many | extremely small |
| XL10-Gold negative control | Kan | 0 |
