IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week8/Widgetry: Difference between revisions
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=Wednesday: August 13, 2008= | =Wednesday: August 13, 2008= | ||
==RU1012 Light Repressible system experiment== | ==RU1012 Light Repressible system experiment== | ||
Streaked plates. | |||
* Dark | * Dark | ||
** Plate 1: RU1012 w/ plasmids | ** Plate 1: RU1012 w/ plasmids | ||
Revision as of 13:38, 19 August 2008
Goals for this week
- Run co-culture test with DH5α, DH5α/pUC19, and Shewie
- Move chambers to the "dark" to prevent a reaction from happening with light and oxygen
- Test light-repressible system on plates
Monday: August 11, 2008
- cleaned chambers
- moved setup to a cabinet to have system in the "dark"
DH5α, DH5α/pUC19, and Shewie co-culture experiment setup
- Chamber 1: DH5α + wt Shewie + Lactose
- Chamber 2: DH5α + wt Shewie + Lactate (pos. control)
- Chamber 3: DH5α + wt Shewie (neg. control)
- Chamber 4: DH5α/pUC19 + wt Shewie + Lactose
- Chamber 5: DH5α/pUC19 + wt Shewie + Lactate (pos. control)
- Chamber 6: DH5α/pUC19 + wt Shewie (neg. control)
- Chamber 7: wt Shewie + Lactose (neg. control)
- Chamber 8: DH5α + Lactose (neg. control)
- Chamber 9: DH5α/pUC19 + Lactose (neg. control)
Tuesday: August 12, 2008
Wednesday: August 13, 2008
RU1012 Light Repressible system experiment
Streaked plates.
- Dark
- Plate 1: RU1012 w/ plasmids
- Plate 2: RU1012
- Plate 3: Negative control (No bugs)
- Light
- Plate 1: RU1012 w/ plasmids
- Plate 2: RU1012
- Plate 3: Negative control (No bugs)
