IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week9/Chemical and Light: Difference between revisions
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Meng Xiao He (talk | contribs) (New page: =Ligations/transformations results 8/18= Parts to ligated came from 8/17 We used a 2:1 molar ratio of insert to vector for all of the ligations. The ligations were all transformed into TO...) |
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Parts to ligated came from 8/17 | Parts to ligated came from 8/17 | ||
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=Colony PCR 08/18= | |||
The annealing temperature was 55 °C and the elongation time was 2'45'' (we used SUPERmix). | |||
Revision as of 11:46, 18 August 2008
Ligations/transformaions results 8/18
Parts to ligated came from 8/17
We used a 2:1 molar ratio of insert to vector for all of the ligations. The ligations were all transformed into TOP10 cells.
| Plasmid | Number of colonies |
| P105+P1'BB | 2 |
| P105+P3'BB | 102 |
| P101+P105 | 0 |
| P101+P108 | 0 |
| mtrB(BB)+P108 | 20 |
| mtrB(BB)+P116 | 4 |
| mtrB(BB)+P117 | 13 |
Colony PCR 08/18
The annealing temperature was 55 °C and the elongation time was 2'45 (we used SUPERmix).
