IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week9/Chemical and Light: Difference between revisions
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Lanes 2-12: P105+P1'BB | Lanes 2-12: P105+P1'BB | ||
=RE digests 08/18= | |||
We used the Fermantas enzymes and digested for 25'. | |||
Lane 1: 1 kb ladder | |||
Lane 2: P45 cut XP (876; 2079) | |||
Lane 3: P63 cut EX (3284) | |||
Lane 4: P97 cut SP (2091) | |||
Revision as of 12:43, 18 August 2008
Ligations/transformaions results 8/18
Parts to ligated came from 8/17
We used a 2:1 molar ratio of insert to vector for all of the ligations. The ligations were all transformed into TOP10 cells.
| Plasmid | Number of colonies |
| P105+P1'BB | 2 |
| P105+P3'BB | 102 |
| P101+P105 | 0 |
| P101+P108 | 0 |
| mtrB(BB)+P108 | 20 |
| mtrB(BB)+P116 | 4 |
| mtrB(BB)+P117 | 13 |
Colony PCR 08/18
The annealing temperature was 55 °C and the elongation time was 2'45" (we used SUPERmix).
Gel 1
Lane 1: 1 kb ladder
Lanes 2-3: P105+P1'BB
Lanes 4-12: P105+P1'BB
Gel 2
Lane 1: 1 kb ladder
Lanes 2-12: P105+P1'BB
Gel 3
Lane 1: 1 kb ladder
Lanes 2-12: P105+P1'BB
RE digests 08/18
We used the Fermantas enzymes and digested for 25'.
Lane 1: 1 kb ladder
Lane 2: P45 cut XP (876; 2079)
Lane 3: P63 cut EX (3284)
Lane 4: P97 cut SP (2091)
