IGEM:Harvard/2008/Lab Notebooks/Recipes: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 9: | Line 9: | ||
* 0.8% agar | * 0.8% agar | ||
* 2 mM MnO2- Mn(IV) oxide (turns from brown to clear upon reduction to Mn(II)) | * 2 mM MnO2- Mn(IV) oxide (turns from brown to clear upon reduction to Mn(II)) | ||
(LML recipes from Beliaev and Saffarini 1998) | |||
Revision as of 09:21, 19 June 2008
Recipes
LML medium (for anaerobic Shewanella growth)
- 0.02% yeast extract
- 0.01% peptone
- 20 mM lactate
- 10 mM HEPES [pH 7.4]
LML agar (for Shewanella reduction test)
- LML medium
- 0.8% agar
- 2 mM MnO2- Mn(IV) oxide (turns from brown to clear upon reduction to Mn(II))
(LML recipes from Beliaev and Saffarini 1998)
