IGEM:Harvard/2008/Plasmids: Difference between revisions
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| align="center" style="background:#eeeeee;"|'''Size''' | | align="center" style="background:#eeeeee;"|'''Size''' | ||
| align="center" style="background:#eeeeee;"|'''Marker''' | | align="center" style="background:#eeeeee;"|'''Marker''' | ||
| align="center" style="background:#eeeeee;"|'''Miniprepped?''' | |||
| align="center" style="background:#eeeeee;"|'''Culture in Glycerol Stock?''' | |||
|- | |- | ||
| P1 || [http://partsregistry.org/Part:BBa_J23113 J23113]||Low promoter GFP tester||pMB1||35||Kan | | P1 || [http://partsregistry.org/Part:BBa_J23113 J23113]||Low promoter GFP tester||pMB1||35||Kan||Yes||in S1 | ||
|- | |- | ||
| P2 || [http://partsregistry.org/Part:BBa_J23113 J23150]||Medium promoter GFP tester||pMB1||35||Kan | | P2 || [http://partsregistry.org/Part:BBa_J23113 J23150]||Medium promoter GFP tester||pMB1||35||Kan||No|| | ||
|- | |- | ||
| P3 || [http://partsregistry.org/Part:BBa_J23113 J23151]||High promoter GFP tester||pMB1||35||Kan | | P3 || [http://partsregistry.org/Part:BBa_J23113 J23151]||High promoter GFP tester||pMB1||35||Kan||Yes||in S1 | ||
|- | |- | ||
| P4 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pACYC duet]||Low-Medium copy vector||p15a||4008||Cm | | P4 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pACYC duet]||Low-Medium copy vector||p15a||4008||Cm||Yes||in S1 and TOP10 | ||
|- | |- | ||
| P5 || [http://partsregistry.org/Part:pSB3K3 pSB3K3]||Low-Medium copy vector (w/ death gene)||p15a||2750||Kan | | P5 || [http://partsregistry.org/Part:pSB3K3 pSB3K3]||Low-Medium copy vector (w/ death gene)||p15a||2750||Kan||No|| | ||
|- | |- | ||
| P6 || [http://partsregistry.org/Part:BBa-E1010 BBa-E1010]||RFP only (w/ death gene)||pMB1||681||Kan | | P6 || [http://partsregistry.org/Part:BBa-E1010 BBa-E1010]||RFP only (w/ death gene)||pMB1||681||Kan|||| | ||
|- | |- | ||
| P7 || [http://partsregistry.org/Part:pSB1A2 pSB1A2]||GFP only||pMB1||2079||Amp | | P7 || [http://partsregistry.org/Part:pSB1A2 pSB1A2]||GFP only||pMB1||2079||Amp|||| | ||
|- | |- | ||
| P8 || [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]||RFP with LacI promoter||pMB1||1069||Amp | | P8 || [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]||RFP with LacI promoter||pMB1||1069||Amp|||| | ||
|- | |- | ||
| P9 || [http://partsregistry.org/Part:BBa_J04430 BBa_J04430]||GFP with LacI promoter||pMB1||1083||Amp | | P9 || [http://partsregistry.org/Part:BBa_J04430 BBa_J04430]||GFP with LacI promoter||pMB1||1083||Amp|||| | ||
|- | |- | ||
| P10 ||[http://partsregistry.org/Part:BBa_I715038 BBa_I715038]||T7 Polymerase with LacI promoter||pMB1||2878||Amp | | P10 ||[http://partsregistry.org/Part:BBa_I715038 BBa_I715038]||T7 Polymerase with LacI promoter||pMB1||2878||Amp|||| | ||
|- | |- | ||
| P11 || [http://partsregistry.org/Part:BBa_M30109 BBa_M30109]||Light responsive system, dual regulation||pUC19-derived pMB1||4333||Amp/Cm | | P11 || [http://partsregistry.org/Part:BBa_M30109 BBa_M30109]||Light responsive system, dual regulation||pUC19-derived pMB1||4333||Amp/Cm|||| | ||
|- | |- | ||
| P12 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pETDuet-1]||Low copy plasmid||pBR322-derived ColE1||5420||Amp | | P12 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pETDuet-1]||Low copy plasmid||pBR322-derived ColE1||5420||Amp|||| | ||
|- | |- | ||
| P13 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pCDFDuet-1]||Low copy plasmid||CloDF13||3781||Sm | | P13 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pCDFDuet-1]||Low copy plasmid||CloDF13||3781||Sm||Yes||in S1 | ||
|- | |- | ||
| P14 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pCOLADuet-1]||Low copy plasmid||ColA||3719||Kan | | P14 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pCOLADuet-1]||Low copy plasmid||ColA||3719||Kan|||| | ||
|- | |- | ||
| P15 || [http://partsregistry.org/Part:BBa_I13522 BBa_I13522]||GFP with Tet promoter||ColE1||937||Amp | | P15 || [http://partsregistry.org/Part:BBa_I13522 BBa_I13522]||GFP with Tet promoter||ColE1||937||Amp|||| | ||
|- | |- | ||
| P16 || [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]||Tet repressible promoter||pMB1||54||Amp | | P16 || [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]||Tet repressible promoter||pMB1||54||Amp|||| | ||
|- | |- | ||
| P17 || [http://partsregistry.org/Part:BBa_Q04400 BBa_Q04400]||Inverter (TetR w/o promoter and Tet promoter)||OriS, P1 lytic, F1||902||Kan | | P17 || [http://partsregistry.org/Part:BBa_Q04400 BBa_Q04400]||Inverter (TetR w/o promoter and Tet promoter)||OriS, P1 lytic, F1||902||Kan|||| | ||
|- | |- | ||
| P18 || [http://partsregistry.org/Part:BBa_R0051 BBa_R0051]||lambda promoter (cI regulated)||pMB1||49||Amp | | P18 || [http://partsregistry.org/Part:BBa_R0051 BBa_R0051]||lambda promoter (cI regulated)||pMB1||49||Amp|||| | ||
|- | |- | ||
| P19 || [http://partsregistry.org/Part:BBa_I763007 BBa_I763007]||RFP with lambda promoter||pMB1||918||Amp | | P19 || [http://partsregistry.org/Part:BBa_I763007 BBa_I763007]||RFP with lambda promoter||pMB1||918||Amp|||| | ||
|- | |- | ||
| P20 || [http://partsregistry.org/Part:BBa_J04431 BBa_J04431]||GFP (LVA tagged) with Lac promoter||pMB1||1122||Amp, Kan | | P20 || [http://partsregistry.org/Part:BBa_J04431 BBa_J04431]||GFP (LVA tagged) with Lac promoter||pMB1||1122||Amp, Kan|||| | ||
|- | |- | ||
| P21 || [???]||A1 (pACYC)||???||???||??? | | P21 || [???]||A1 (pACYC)||???||???||???||Yes||in S1 and TOP10 | ||
|- | |- | ||
| P22 || [http://partsregistry.org/Part:BBa_R0082 BBa_R0082]||ompR controlled promoter for ompC (long)||pMB1||108||Amp | | P22 || [http://partsregistry.org/Part:BBa_R0082 BBa_R0082]||ompR controlled promoter for ompC (long)||pMB1||108||Amp|||| | ||
|- | |- | ||
| P23 || [http://partsregistry.org/Part:BBa_R0083 BBa_R0083]||ompR controlled promoter for ompC (short)||pMB1||78||Amp | | P23 || [http://partsregistry.org/Part:BBa_R0083 BBa_R0083]||ompR controlled promoter for ompC (short)||pMB1||78||Amp|||| | ||
|- | |- | ||
| P24 || [http://partsregistry.org/Part:BBa_R0084 BBa_R0084]||ompR controlled promoter for ompF||pMB1||108||Amp | | P24 || [http://partsregistry.org/Part:BBa_R0084 BBa_R0084]||ompR controlled promoter for ompF||pMB1||108||Amp|||| | ||
|- | |- | ||
| P25 || [http://partsregistry.org/Part:BBa_J58102 BBa_J58102]||Promoter activated by OmpR-P with the reporter GFP||pUC19-derived pMB1||991||Amp | | P25 || [http://partsregistry.org/Part:BBa_J58102 BBa_J58102]||Promoter activated by OmpR-P with the reporter GFP||pUC19-derived pMB1||991||Amp|||| | ||
|- | |- | ||
| P26 || [http://partsregistry.org/Part:BBa_J58103 BBa_J58103]||Mutated promoter activated by OmpR-P with the reporter GFP||pUC19-derived pMB1||961||Amp | | P26 || [http://partsregistry.org/Part:BBa_J58103 BBa_J58103]||Mutated promoter activated by OmpR-P with the reporter GFP||pUC19-derived pMB1||961||Amp|||| | ||
|- | |- | ||
| P27 ||pZS4int2||repressor plasmid||pSC101||||Sm | | P27 ||pZS4int2||repressor plasmid||pSC101||||Sm|||| | ||
|- | |- | ||
| P28 ||pZE21||GFP||ColE1||||Kan | | P28 ||pZE21||GFP||ColE1||||Kan|||| | ||
|- | |- | ||
| P29 ||pZS*2R||Venus (mutant YFP)||pSC101*||||Kan | | P29 ||pZS*2R||Venus (mutant YFP)||pSC101*||||Kan|||| | ||
|- | |- | ||
| P30 ||pZA32||YFP||p15A||||Cm | | P30 ||pZA32||YFP||p15A||||Cm|||| | ||
|- | |- | ||
| P31 ||pZS*1R||Venus||pSC101*||||Amp | | P31 ||pZS*1R||Venus||pSC101*||||Amp|||| | ||
|- | |- | ||
| P32 ||pZA32||Venus||p15A||||Cm | | P32 ||pZA32||Venus||p15A||||Cm|||| | ||
|- | |- | ||
| P33 ||pZA31||Venus||p15A||||Cm | | P33 ||pZA31||Venus||p15A||||Cm|||| | ||
|- | |- | ||
| P34 ||pZE12||GFP||ColE1||||Amp | | P34 ||pZE12||GFP||ColE1||||Amp|||| | ||
|- | |- | ||
| P35 ||pLDR8|||||||| | | P35 ||pLDR8|||||||||||| | ||
|- | |- | ||
| P36 || [http://partsregistry.org/Part:BBa_I763004 BBa_I763004]||GFP + IPTG + LVA||||||Kan/Amp | | P36 || [http://partsregistry.org/Part:BBa_I763004 BBa_I763004]||GFP + IPTG + LVA||||||Kan/Amp|| | ||
|} | |} |
Revision as of 13:46, 26 June 2008
Vectors
Parts
Name | Registry Name | Description | Origin | Size | Marker | Miniprepped? | Culture in Glycerol Stock? |
P1 | J23113 | Low promoter GFP tester | pMB1 | 35 | Kan | Yes | in S1 |
P2 | J23150 | Medium promoter GFP tester | pMB1 | 35 | Kan | No | |
P3 | J23151 | High promoter GFP tester | pMB1 | 35 | Kan | Yes | in S1 |
P4 | pACYC duet | Low-Medium copy vector | p15a | 4008 | Cm | Yes | in S1 and TOP10 |
P5 | pSB3K3 | Low-Medium copy vector (w/ death gene) | p15a | 2750 | Kan | No | |
P6 | BBa-E1010 | RFP only (w/ death gene) | pMB1 | 681 | Kan | ||
P7 | pSB1A2 | GFP only | pMB1 | 2079 | Amp | ||
P8 | BBa_J04450 | RFP with LacI promoter | pMB1 | 1069 | Amp | ||
P9 | BBa_J04430 | GFP with LacI promoter | pMB1 | 1083 | Amp | ||
P10 | BBa_I715038 | T7 Polymerase with LacI promoter | pMB1 | 2878 | Amp | ||
P11 | BBa_M30109 | Light responsive system, dual regulation | pUC19-derived pMB1 | 4333 | Amp/Cm | ||
P12 | pETDuet-1 | Low copy plasmid | pBR322-derived ColE1 | 5420 | Amp | ||
P13 | pCDFDuet-1 | Low copy plasmid | CloDF13 | 3781 | Sm | Yes | in S1 |
P14 | pCOLADuet-1 | Low copy plasmid | ColA | 3719 | Kan | ||
P15 | BBa_I13522 | GFP with Tet promoter | ColE1 | 937 | Amp | ||
P16 | BBa_R0040 | Tet repressible promoter | pMB1 | 54 | Amp | ||
P17 | BBa_Q04400 | Inverter (TetR w/o promoter and Tet promoter) | OriS, P1 lytic, F1 | 902 | Kan | ||
P18 | BBa_R0051 | lambda promoter (cI regulated) | pMB1 | 49 | Amp | ||
P19 | BBa_I763007 | RFP with lambda promoter | pMB1 | 918 | Amp | ||
P20 | BBa_J04431 | GFP (LVA tagged) with Lac promoter | pMB1 | 1122 | Amp, Kan | ||
P21 | [???] | A1 (pACYC) | ??? | ??? | ??? | Yes | in S1 and TOP10 |
P22 | BBa_R0082 | ompR controlled promoter for ompC (long) | pMB1 | 108 | Amp | ||
P23 | BBa_R0083 | ompR controlled promoter for ompC (short) | pMB1 | 78 | Amp | ||
P24 | BBa_R0084 | ompR controlled promoter for ompF | pMB1 | 108 | Amp | ||
P25 | BBa_J58102 | Promoter activated by OmpR-P with the reporter GFP | pUC19-derived pMB1 | 991 | Amp | ||
P26 | BBa_J58103 | Mutated promoter activated by OmpR-P with the reporter GFP | pUC19-derived pMB1 | 961 | Amp | ||
P27 | pZS4int2 | repressor plasmid | pSC101 | Sm | |||
P28 | pZE21 | GFP | ColE1 | Kan | |||
P29 | pZS*2R | Venus (mutant YFP) | pSC101* | Kan | |||
P30 | pZA32 | YFP | p15A | Cm | |||
P31 | pZS*1R | Venus | pSC101* | Amp | |||
P32 | pZA32 | Venus | p15A | Cm | |||
P33 | pZA31 | Venus | p15A | Cm | |||
P34 | pZE12 | GFP | ColE1 | Amp | |||
P35 | pLDR8 | ||||||
P36 | BBa_I763004 | GFP + IPTG + LVA | Kan/Amp |