IGEM:Harvard/2008/Plasmids: Difference between revisions

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==Parts==
==Parts==
*[http://spreadsheets.google.com/ccc?key=pwvxAM8xwIq37aJ-qKhmUag&hl=en Google Spreadsheet]
{| {{table}}
{| {{table}}
| align="center" style="background:#eeeeee;"|'''Name'''
| align="center" style="background:#eeeeee;"|'''Name'''
Line 11: Line 12:
| align="center" style="background:#eeeeee;"|'''Size'''
| align="center" style="background:#eeeeee;"|'''Size'''
| align="center" style="background:#eeeeee;"|'''Marker'''
| align="center" style="background:#eeeeee;"|'''Marker'''
 
| align="center" style="background:#eeeeee;"|'''Transformed?'''
| align="center" style="background:#eeeeee;"|'''Miniprepped?'''
| align="center" style="background:#eeeeee;"|'''Culture in Glycerol Stock?'''
| align="center" style="background:#eeeeee;"|'''Sequenced?'''
|-
|-
| P1 || [http://partsregistry.org/Part:BBa_J23113 J23113]||Low promoter GFP tester||pMB1||35||Kan
| P1 || [http://partsregistry.org/Part:BBa_I20259 J23113/I20259]||Low promoter GFP tester||p15A pMR101-derived||919||Kan||in S1 and TOP10||Yes for both strains|| Yes for both strains.||||
|-
|-
| P2 || [http://partsregistry.org/Part:BBa_J23113 J23150]||Medium promoter GFP tester||pMB1||35||Kan
| P2 || [http://partsregistry.org/Part:BBa_I20269 J23150/I20269]||Medium promoter GFP tester||p15A pMR101-derived||919||Kan||in TOP10, not S1||||
|-
|-
| P3 || [http://partsregistry.org/Part:BBa_J23113 J23151]||High promoter GFP tester||pMB1||35||Kan
| P3 || [http://partsregistry.org/Part:BBa_I20270 J23151/I20270]||High promoter GFP tester||p15A pMR101-derived||919||Kan||in TOP10 and S1||Yes for both strains.||Yes for both strains.||
|-
|-
| P4 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pACYC duet]||Low-Medium copy vector||p15a||4008||Cm
| P4 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pACYC duet]||Low-Medium copy vector||p15a||4008||Cm||Yes||in S1 and TOP10||||
|-
|-
| P5 || [http://partsregistry.org/Part:pSB3K3 pSB3K3]||Low-Medium copy vector||p15a||2750||Kan
| P5 || [http://partsregistry.org/Part:pSB3K3 pSB3K3]||Low-Medium copy vector (w/ death gene)||p15a||2750||Kan||||||
|-
|-
| P6 || [http://partsregistry.org/Part:BBa-E1010 BBa-E1010]||RFP only||pMB1||681||Kan
| P6 || [http://partsregistry.org/Part:BBa-E1010 BBa-E1010]||RFP only (w/ death gene)||pMB1||681||Kan||||||
|-
|-
| P7 || [http://partsregistry.org/Part:pSB1A2 pSB1A2]||GFP only||pMB1||2079||Amp
| P7 || [http://partsregistry.org/Part:pSB1A2 pSB1A2]||GFP only||pMB1||2079||Amp||||||
|-
|-
| P8 || [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]||RFP with LacI promoter||pMB1||1069||Amp
| P8 || [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]||RFP with LacI promoter||pMB1||1069||Amp||||||
|-
|-
| P9 || [http://partsregistry.org/Part:BBa_J04430 BBa_J04430]||GFP with LacI promoter||pMB1||1083||Amp
| P9 || [http://partsregistry.org/Part:BBa_J04430 BBa_J04430]||GFP with LacI promoter||pMB1||1083||Amp||||||
|-
|-
| P10 ||[http://partsregistry.org/Part:BBa_I715038 BBa_I715038]||T7 Polymerase with LacI promoter||pMB1||2878||Amp
| P10 ||[http://partsregistry.org/Part:BBa_I715038 BBa_I715038]||T7 Polymerase with LacI promoter||pMB1||2878||Amp||||||
|-
|-
| P11 || [http://partsregistry.org/Part:BBa_M30109 BBa_M30109]||Light responsive system, dual regulation||pUC19-derived pMB1||4333||Amp/Cm
| P11 || [http://partsregistry.org/Part:BBa_M30109 BBa_M30109]||Light responsive system, dual regulation||pUC19-derived pMB1||4333||Amp/Cm||||||
|-
|-
| P15 || [http://partsregistry.org/Part:BBa_I13522 BBa_I13522]||GFP with Tet promoter||ColE1||937||Amp
| P12 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pETDuet-1]||Low copy plasmid||pBR322-derived ColE1||5420||Amp||||||
|-
|-
| P16 || [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]||Tet repressible promoter||pMB1||54||Amp
| P13 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pCDFDuet-1]||Low copy plasmid||CloDF13||3781||Sm||Yes||in S1||
|-
|-
| P17 || [http://partsregistry.org/Part:BBa_Q04400 BBa_Q04400]||Inverter (TetR w/o promoter and Tet promoter)||OriS, P1 lytic, F1||902||Kan
| P14 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pCOLADuet-1]||Low copy plasmid||ColA||3719||Kan||||||
|-
| P15 || [http://partsregistry.org/Part:BBa_I13522 BBa_I13522]||GFP with Tet promoter||ColE1||937||Amp||||||
|-
| P16 || [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]||Tet repressible promoter||pMB1||54||Amp||||||
|-
| P17 || [http://partsregistry.org/Part:BBa_Q04400 BBa_Q04400]||Inverter (TetR w/o promoter and Tet promoter)||OriS, P1 lytic, F1||902||Kan||||||
|-
| P18 || [http://partsregistry.org/Part:BBa_R0051 BBa_R0051]||lambda promoter (cI regulated)||pMB1||49||Amp||||||
|-
| P19 || [http://partsregistry.org/Part:BBa_I763007 BBa_I763007]||RFP with lambda promoter||pMB1||918||Amp||||||
|-
| P20 || [http://partsregistry.org/Part:BBa_J04431 BBa_J04431]||GFP (LVA tagged) with Lac promoter||pMB1||1122||Amp, Kan||||||
|-
| P21 ||A1 (pACYC)||pACYC (P4) w/ biobricks||???||???||???||Yes||in S1 and TOP10||
|-
| P22 || [http://partsregistry.org/Part:BBa_R0082 BBa_R0082]||ompR controlled promoter for ompC (long)||pMB1||108||Amp||||||
|-
| P23 || [http://partsregistry.org/Part:BBa_R0083 BBa_R0083]||ompR controlled promoter for ompC (short)||pMB1||78||Amp||||||
|-
| P24 || [http://partsregistry.org/Part:BBa_R0084 BBa_R0084]||ompR controlled promoter for ompF||pMB1||108||Amp||||||
|-
| P25 || [http://partsregistry.org/Part:BBa_J58102 BBa_J58102]||Promoter activated by OmpR-P with the reporter GFP||pUC19-derived pMB1||991||Amp||||||
|-
| P26 || [http://partsregistry.org/Part:BBa_J58103 BBa_J58103]||Mutated promoter activated by OmpR-P with the reporter GFP||pUC19-derived pMB1||961||Amp||||||
|-
| P27 ||pZS4int2||repressor plasmid||pSC101||||Sm||Yes||||
|-
| P28 ||pZE21||GFP||ColE1||||Kan||Yes||||
|-
| P29 ||pZS*2R||Venus (mutant YFP)||pSC101*||||Kan||Yes||||
|-
| P30 ||pZA32||YFP||p15A||||Cm||Yes||||
|-
| P31 ||pZS*1R||Venus||pSC101*||||Amp||Yes||||
|-
| P32 ||pZA32||Venus||p15A||||Cm||Yes||||
|-
| P33 ||pZA31||Venus||p15A||||Cm||Yes||||
|-
| P34 ||pZE12||GFP||ColE1||||Amp||Yes||||
|-
| P35 ||pLDR8||||||||||Yes||||
|-
| P36 || [http://partsregistry.org/Part:BBa_I763004 BBa_I763004]||GFP + IPTG + LVA||||||Kan/Amp||||||
|-
| P37 || [http://partsregistry.org/wiki/index.php/Part:BBa_I722007 BBa_I722007]||Constructive expression LacI w/ pTetR promoter||||||Amp||||||
|-
| P38 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23114 BBa_J23114]||High constitutive promoter||||||Amp||||||
|-
| P39 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23113 BBa_J23113]||Low constitutive promoter||||||Amp||||||
|-
| P40 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032]||Ribosome Binding Site||||||Amp||||||
|-
| P41 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0010 BBa_B0010]||Transcriptional Terminator||||||Amp||||||
|-
| P42 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012]||LacI coding region||||||Amp||||||
|-
| P43 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040]||TetR coding region||||||Amp||||||
|-
| P44 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 BBa_C0051]||cI lambda||||||Amp||||||
|-
| P45 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 BBa_E0240]||GFP only w/ RBS & terminator||||||Amp||||||
|-
| P46 || [http://partsregistry.org/Part:BBa_I51020 BBa_I51020]||Base Vector|||||||Amp||||||
|-
| P47 || [http://partsregistry.org/Part:BBa_P1003 BBa_P1003]||Kan Resistance Cassette||||||Kan||||||
|-
| P48 || [http://partsregistry.org/Part:BBa_P1004 BBa_P1004]||Cm Resistance Cassette||||||Cm||||||
|-
| P49 || [http://partsregistry.org/Part:BBa_P1002 BBa_P1002]||Amp Resistance Cassette|||||||Amp||||||
|-
| P50 || [http://partsregistry.org/Part:BBa_P1005 BBa_P1005]||Tet Resistance Cassette||||||Tet||||||
|-
| P51 || [http://partsregistry.org/Part:BBa_Q04121 BBa_Q04121]||LacI QPI w/ RBS & promoter||||||Kan||||||
|-
| P52 || [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]||cI Lambda QPI||||||Kan||||||
|-
| P53 || [http://partsregistry.org/Part:BBa_P0440 BBa_P0440]||PoPS -> TetR||||||Amp||||||
|-
| P54 || [http://partsregistry.org/Part:BBa_P0412 BBa_P0412]||PoPS -> LacI||||||Amp||||||
|-
| P55 || [http://partsregistry.org/Part:BBa_P0455 BBa_P0455]||RBS.cI.LVA.Term||||||Amp/Cm||||||
|}
|}

Latest revision as of 12:16, 1 July 2008

Vectors

Parts

Name Registry Name Description Origin Size Marker Transformed? Miniprepped? Culture in Glycerol Stock? Sequenced?
P1 J23113/I20259 Low promoter GFP tester p15A pMR101-derived 919 Kan in S1 and TOP10 Yes for both strains Yes for both strains.
P2 J23150/I20269 Medium promoter GFP tester p15A pMR101-derived 919 Kan in TOP10, not S1
P3 J23151/I20270 High promoter GFP tester p15A pMR101-derived 919 Kan in TOP10 and S1 Yes for both strains. Yes for both strains.
P4 pACYC duet Low-Medium copy vector p15a 4008 Cm Yes in S1 and TOP10
P5 pSB3K3 Low-Medium copy vector (w/ death gene) p15a 2750 Kan
P6 BBa-E1010 RFP only (w/ death gene) pMB1 681 Kan
P7 pSB1A2 GFP only pMB1 2079 Amp
P8 BBa_J04450 RFP with LacI promoter pMB1 1069 Amp
P9 BBa_J04430 GFP with LacI promoter pMB1 1083 Amp
P10 BBa_I715038 T7 Polymerase with LacI promoter pMB1 2878 Amp
P11 BBa_M30109 Light responsive system, dual regulation pUC19-derived pMB1 4333 Amp/Cm
P12 pETDuet-1 Low copy plasmid pBR322-derived ColE1 5420 Amp
P13 pCDFDuet-1 Low copy plasmid CloDF13 3781 Sm Yes in S1
P14 pCOLADuet-1 Low copy plasmid ColA 3719 Kan
P15 BBa_I13522 GFP with Tet promoter ColE1 937 Amp
P16 BBa_R0040 Tet repressible promoter pMB1 54 Amp
P17 BBa_Q04400 Inverter (TetR w/o promoter and Tet promoter) OriS, P1 lytic, F1 902 Kan
P18 BBa_R0051 lambda promoter (cI regulated) pMB1 49 Amp
P19 BBa_I763007 RFP with lambda promoter pMB1 918 Amp
P20 BBa_J04431 GFP (LVA tagged) with Lac promoter pMB1 1122 Amp, Kan
P21 A1 (pACYC) pACYC (P4) w/ biobricks ??? ??? ??? Yes in S1 and TOP10
P22 BBa_R0082 ompR controlled promoter for ompC (long) pMB1 108 Amp
P23 BBa_R0083 ompR controlled promoter for ompC (short) pMB1 78 Amp
P24 BBa_R0084 ompR controlled promoter for ompF pMB1 108 Amp
P25 BBa_J58102 Promoter activated by OmpR-P with the reporter GFP pUC19-derived pMB1 991 Amp
P26 BBa_J58103 Mutated promoter activated by OmpR-P with the reporter GFP pUC19-derived pMB1 961 Amp
P27 pZS4int2 repressor plasmid pSC101 Sm Yes
P28 pZE21 GFP ColE1 Kan Yes
P29 pZS*2R Venus (mutant YFP) pSC101* Kan Yes
P30 pZA32 YFP p15A Cm Yes
P31 pZS*1R Venus pSC101* Amp Yes
P32 pZA32 Venus p15A Cm Yes
P33 pZA31 Venus p15A Cm Yes
P34 pZE12 GFP ColE1 Amp Yes
P35 pLDR8 Yes
P36 BBa_I763004 GFP + IPTG + LVA Kan/Amp
P37 BBa_I722007 Constructive expression LacI w/ pTetR promoter Amp
P38 BBa_J23114 High constitutive promoter Amp
P39 BBa_J23113 Low constitutive promoter Amp
P40 BBa_B0032 Ribosome Binding Site Amp
P41 BBa_B0010 Transcriptional Terminator Amp
P42 BBa_C0012 LacI coding region Amp
P43 [1] TetR coding region Amp
P44 BBa_C0051 cI lambda Amp
P45 BBa_E0240 GFP only w/ RBS & terminator Amp
P46 BBa_I51020 Base Vector Amp
P47 BBa_P1003 Kan Resistance Cassette Kan
P48 BBa_P1004 Cm Resistance Cassette Cm
P49 BBa_P1002 Amp Resistance Cassette Amp
P50 BBa_P1005 Tet Resistance Cassette Tet
P51 BBa_Q04121 LacI QPI w/ RBS & promoter Kan
P52 BBa_Q04510 cI Lambda QPI Kan
P53 BBa_P0440 PoPS -> TetR Amp
P54 BBa_P0412 PoPS -> LacI Amp
P55 BBa_P0455 RBS.cI.LVA.Term Amp/Cm
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