IGEM:Harvard/2008/Plasmids

Vectors

• pACYC-Duet vector map
• pSB3K3

Parts

Name	Registry Name	Description	Origin	Size	Marker
P1	J23113	Low promoter GFP tester	pMB1	35	Kan
P2	J23150	Medium promoter GFP tester	pMB1	35	Kan
P3	J23151	High promoter GFP tester	pMB1	35	Kan
P4	pACYC duet	Low-Medium copy vector	p15a	4008	Cm
P5	pSB3K3	Low-Medium copy vector (w/ death gene)	p15a	2750	Kan
P6	BBa-E1010	RFP only (w/ death gene)	pMB1	681	Kan
P7	pSB1A2	GFP only	pMB1	2079	Amp
P8	BBa_J04450	RFP with LacI promoter	pMB1	1069	Amp
P9	BBa_J04430	GFP with LacI promoter	pMB1	1083	Amp
P10	BBa_I715038	T7 Polymerase with LacI promoter	pMB1	2878	Amp
P11	BBa_M30109	Light responsive system, dual regulation	pUC19-derived pMB1	4333	Amp/Cm
P12	pETDuet-1	Low copy plasmid	pBR322-derived ColE1	5420	Amp
P13	pCDFDuet-1	Low copy plasmid	CloDF13	3781	Sm
P14	pCOLADuet-1	Low copy plasmid	ColA	3719	Kan
P15	BBa_I13522	GFP with Tet promoter	ColE1	937	Amp
P16	BBa_R0040	Tet repressible promoter	pMB1	54	Amp
P17	BBa_Q04400	Inverter (TetR w/o promoter and Tet promoter)	OriS, P1 lytic, F1	902	Kan
P18	BBa_R0051	lambda promoter (cI regulated)	pMB1	49	Amp
P19	BBa_I763007	RFP with lambda promoter	pMB1	918	Amp
P20	BBa_J04431	GFP (LVA tagged) with Lac promoter	pMB1	1122	Amp, Kan
P21	[???]	A1 (pACYC)	???	???	???
P22	BBa_R0082	ompR controlled promoter for ompC (long)	pMB1	108	Amp
P23	BBa_R0083	ompR controlled promoter for ompC (short)	pMB1	78	Amp
P24	BBa_R0084	ompR controlled promoter for ompF	pMB1	108	Amp
P25	BBa_J58102	Promoter activated by OmpR-P with the reporter GFP	pUC19-derived pMB1	991	Amp
P26	BBa_J58103	Mutated promoter activated by OmpR-P with the reporter GFP	pUC19-derived pMB1	961	Amp
P27	pZS4int2	repressor plasmid	pSC101		Sm
P28	pZE21	GFP	ColE1		Kan
P29	pZS*2R	Venus (mutant YFP)	pSC101*		Kan
P30	pZA32	YFP	p15A		Cm
P31	pZS*1R	Venus	pSC101*		Amp
P32	pZA32	Venus	p15A		Cm
P33	pZA31	Venus	p15A		Cm
P34	pZE12	GFP	ColE1		Amp
P35	pLDR8
P36	BBa_I763004	GFP + IPTG + LVA			Kan/Amp