IGEM:Harvard/2008/Questions for Orianna Bretschger: Difference between revisions
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(New page: =Questions= General * Transformation protocol (not via conjugation) * Plasmids that can be replicated (origins) * Promoters that work (inducible, etc) * Request for gifts * Can mtr genes b...) |
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Revision as of 13:23, 19 June 2008
Questions
General
- Transformation protocol (not via conjugation)
- Plasmids that can be replicated (origins)
- Promoters that work (inducible, etc)
- Request for gifts
- Can mtr genes be regulated (eg lacZ)?
Knocking out EnvZ and/or OmpR (together a bit over 2kbp)
- Homologous recombination- time frame, ease
- Cre-lox- which one is easier?
