IGEM:Harvard/2008/Strains: Difference between revisions

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== '''Strains''' ==
== '''Strains''' ==


* ''Shewanella putrefaciens'' CN-32
* S1: ''Shewanella oneidensis'' MR-1
** [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=211586 Codon usage]
**[[Shewanella oneidensis 16s rRNA]]
**Colonies go slightly pink as they reach maturity. Pellets of overnights are pink. Is prob. high heme content in anaerobic conditions.
* S2: ''Shewanella putrefaciens'' CN-32
** [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=319224 Codon usage]
** [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=319224 Codon usage]
* ''Shewanella oneidensis'' MR-1
** [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=211586 Codon usage]
** [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=700550&Template=bacteria ATCC page - growth conditions]
** [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=700550&Template=bacteria ATCC page - growth conditions]
* ''Escherichia coli'' K12
* S3: ''Shewanella oneidensis MR-1'' ΔmtrA
** [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=83333 Codon usage]
* S4: ''Shewanella oneidensis MR-1'' ΔmtrB
** [[/DH5a-J23113/]] Low GFP reference - (note: DH5a, not Top10)
* S5: ''Snewanella oneidensis MR-1'' ΔmtrC/omcA
** [[/DH5a-J23150/]] Med GFP reference - (note: DH5a, not Top10)
* E1: Escherichia coli DH5α
** [[/DH5a-J23151/]] High GFP reference - (note: DH5a, not Top10, and not yet in...)
**[[16s rRNA (for DH10B)]]
 
* E2: Escherichia coli TOP10
* E3: Escherichia coli DB31 (ccDB res) (death-resistant strain)
* E4: RU1012 [MC4100 ara+ Φ(OmpC-lacZ) 10-25 ΔenvZ::KanR]
* E5: XL1-Blue (from QuikChange kit)


== '''Growth conditions''' ==
== '''Growth conditions''' ==
* [[/E coli/]]
* [[/E coli/]]
* [[/Shewy/]]
* [[/Shewie/]]

Latest revision as of 17:29, 13 August 2008

Yeah, this will need some reorganization...


Strains

  • S1: Shewanella oneidensis MR-1
  • S2: Shewanella putrefaciens CN-32
  • S3: Shewanella oneidensis MR-1 ΔmtrA
  • S4: Shewanella oneidensis MR-1 ΔmtrB
  • S5: Snewanella oneidensis MR-1 ΔmtrC/omcA
  • E1: Escherichia coli DH5α
  • E2: Escherichia coli TOP10
  • E3: Escherichia coli DB31 (ccDB res) (death-resistant strain)
  • E4: RU1012 [MC4100 ara+ Φ(OmpC-lacZ) 10-25 ΔenvZ::KanR]
  • E5: XL1-Blue (from QuikChange kit)

Growth conditions

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