IGEM:Harvard/2009/Lab Notebook/Week1: Difference between revisions
Amrita Goyal (talk | contribs) |
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=Tuesday: June 16, 2009= | =Tuesday: June 16, 2009= | ||
Since we are still unable to obtain PhyA, PhyB, and PIF3 cDNAs from any of the sources we have enquired with (although there is a possible lead from the Mathews group), we have decided to pursue extraction of mRNA from ''Arabidopsis'' directly. We obtained two small Colombian ''Arabidopsis'' plants from the Pierce lab, courtesy of one of their graduate students, and will use the Qiagen Plant RNeasy kit for mRNA extraction. Per their instructions, we should flash freeze the leaves with liquid nitrogen, grind them with a frozen mortor and pestal under liquid nitrogen, and then use the kit for the extraction. | |||
As of the moment we are still waiting to hear back from the Mathews group about a cDNA library or PhyA and PhyC cDNAs; a phone call was placed to them today. Hopefully they should get back to us tomorrow. | |||
-AG | |||
=Wednesday: June 17, 2009= | =Wednesday: June 17, 2009= | ||
Revision as of 11:06, 16 June 2009
Monday: June 15, 2009
Tuesday: June 16, 2009
Since we are still unable to obtain PhyA, PhyB, and PIF3 cDNAs from any of the sources we have enquired with (although there is a possible lead from the Mathews group), we have decided to pursue extraction of mRNA from Arabidopsis directly. We obtained two small Colombian Arabidopsis plants from the Pierce lab, courtesy of one of their graduate students, and will use the Qiagen Plant RNeasy kit for mRNA extraction. Per their instructions, we should flash freeze the leaves with liquid nitrogen, grind them with a frozen mortor and pestal under liquid nitrogen, and then use the kit for the extraction.
As of the moment we are still waiting to hear back from the Mathews group about a cDNA library or PhyA and PhyC cDNAs; a phone call was placed to them today. Hopefully they should get back to us tomorrow.
-AG
