IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/17: Difference between revisions
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Following QIAgen PCR Purification Kit Protocol the following PCR products were purified: | Following QIAgen PCR Purification Kit Protocol the following PCR products were purified: | ||
#pENTCUP2 Promoter | #pENTCUP2 Promoter - 16.1 ng/μL | ||
#NOSterminator Sequence | #NOSterminator Sequence - 76.3 ng/μL | ||
#STOP codon + NOSterminator Sequence | #STOP codon + NOSterminator Sequence - 76.5 ng/μL | ||
To do: follow up with restriction digest <font color="green"> ✓ </font> and Agarose gel to confirm PCR and PCR purification. <!--<font color="green"> ✓ </font>--> | To do: follow up with restriction digest <font color="green"> ✓ </font> and Agarose gel to confirm PCR and PCR purification. <!--<font color="green"> ✓ </font>--> | ||
Revision as of 09:28, 17 June 2010
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Team FlavourPCR Purification of pORE Vector PartsFollowing QIAgen PCR Purification Kit Protocol the following PCR products were purified:
To do: follow up with restriction digest ✓ and Agarose gel to confirm PCR and PCR purification.
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