IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/17: Difference between revisions
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Restriction Digest reactions were set up as follows: | Restriction Digest reactions were set up as follows: | ||
pENTCUP2 Promoter: | pENTCUP2 Promoter:<br> | ||
27μL PCR product | *27μL PCR product<br> | ||
3μL Loading Buffer w/ dye | *3μL Loading Buffer w/ dye<br> | ||
1μL Xba1 Fast Enzyme | *1μL Xba1 Fast Enzyme<br> | ||
1μL Pst1 Fast Enzyme | *1μL Pst1 Fast Enzyme<br> | ||
NOSterm and NOSterm + STOP: | NOSterm and NOSterm + STOP: | ||
9μL PCR product | *9μL PCR product | ||
1μL Loading Buffer w/ dye | *1μL Loading Buffer w/ dye | ||
0.5μL Xba1 Fast Enzyme | *0.5μL Xba1 Fast Enzyme | ||
0.5μL Pst1 Fast Enzyme | *0.5μL Pst1 Fast Enzyme | ||
BioBrick Plasmid V0120: | BioBrick Plasmid V0120: | ||
3μL DNA | *3μL DNA | ||
1μL Loading Buffer w/ dye | *1μL Loading Buffer w/ dye | ||
6μL diH<sub>2</sub>O | *6μL diH<sub>2</sub>O | ||
0.5μL Xba1 Fast Enzyme | *0.5μL Xba1 Fast Enzyme | ||
0.5μL Pst1 Fast Enzyme | *0.5μL Pst1 Fast Enzyme | ||
Revision as of 11:07, 17 June 2010
 iGEM Project name 1
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Team FlavourPCR Purification of pORE Vector PartsFollowing QIAgen PCR Purification Kit Protocol the following PCR products were purified:
To do: follow up with restriction digest ✓ and Agarose gel to confirm PCR and PCR purification. Restriction DigestRestriction Digest reactions were set up as follows: pENTCUP2 Promoter:
NOSterm and NOSterm + STOP:
BioBrick Plasmid V0120:
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