IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/17: Difference between revisions
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#NOSterminator Sequence - 76.3 ng/μL | #NOSterminator Sequence - 76.3 ng/μL | ||
#STOP codon + NOSterminator Sequence - 76.5 ng/μL | #STOP codon + NOSterminator Sequence - 76.5 ng/μL | ||
To do: follow up with restriction digest <font color="green"> ✓ </font> and Agarose gel to confirm PCR and PCR purification. <!--<font color="green"> ✓ </font>--> | To do: follow up with restriction digest <font color="green"> ✓ </font> and Agarose gel<font color="green"> ✓ </font> to confirm PCR and PCR purification. <!--<font color="green"> ✓ </font>--> | ||
===Restriction Digest=== | ===Restriction Digest=== | ||
Revision as of 11:08, 17 June 2010
 iGEM Project name 1
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Team FlavourPCR Purification of pORE Vector PartsFollowing QIAgen PCR Purification Kit Protocol the following PCR products were purified:
To do: follow up with restriction digest ✓ and Agarose gel ✓ to confirm PCR and PCR purification. Restriction DigestRestriction Digest reactions were set up as follows: pENTCUP2 Promoter:
NOSterm and NOSterm + STOP:
BioBrick Plasmid V0120:
Reactions were allowed to proceed for 45 minutes at 37°C. | |
