IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/18

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(Team Vector)
(Team Vector)
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==Team Vector==
==Team Vector==
[[Image:PORE_Vectors.xls]]
[[Image:PORE_Vectors.xls]]
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<html>
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Aim: To biobrick agrobacteria vectors<br /><br />
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We obtained the following pORE agrobacteria vectors from TAIR.<br /><br />
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<table>
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<tr>
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<td>Name</td>
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<td>Original Name</td>
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<td>Description</td>
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<td>Plant Resistance</td>
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</tr>
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<tr>
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<td>V1</td>
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<td>pORE_O1</td>
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<td>Agrobacterium vector open series</td>
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<td>pat</td>
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</tr>
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<tr>
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<td>V2</td>
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<td>pORE_O2</td>
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<td>Agrobacterium vector open series</td>
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<td>nptll</td>
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</tr>
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<tr>
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<td>V3</td>
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<td>pORE_E3</td>
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<td>Agrobacterium vector with ENTCUP2 promoter</td>
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<td>pat</td>
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</tr>
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<tr>
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<td>V4</td>
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<td>pORE_E4</td>
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<td>Agrobacterium vector with ENTCUP2 promoter</td>
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<td>nptll</td>
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</tr>
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<tr>
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<td>V5</td>
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<td>pORE_R1</td>
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<td>Agrobacterium vector with gusA reporter</td>
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<td>nptll</td>
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</tr>
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<tr>
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<td>V6</td>
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<td>pORE_R3</td>
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<td>Agrobacterium vector with smgfp reporter</td>
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<td>nptll</td>
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</tr>
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</table>
==Team Flavor==
==Team Flavor==

Revision as of 15:09, 18 June 2010

iGEM iGarden Main project page
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Week 1 Summary (6/18/2010)

Team Vector

Image:PORE Vectors.xls

Aim: To biobrick agrobacteria vectors

We obtained the following pORE agrobacteria vectors from TAIR.

Name Original Name Description Plant Resistance
V1 pORE_O1 Agrobacterium vector open series pat
V2 pORE_O2 Agrobacterium vector open series nptll
V3 pORE_E3 Agrobacterium vector with ENTCUP2 promoter pat
V4 pORE_E4 Agrobacterium vector with ENTCUP2 promoter nptll
V5 pORE_R1 Agrobacterium vector with gusA reporter nptll
V6 pORE_R3 Agrobacterium vector with smgfp reporter nptll
==Team Flavor== ==Team Allergy== '''Progress''' # Identified allergens in arabidopsis, strawberries,tomatoes, and carrots and created primers for amplifying out these allergens [http://openwetware.org/wiki/IGEM:Harvard/2010/Team_Allergy| (see primers and sequences)] # Extracted cDNA (rna extraction followed by rt-pcr) from strawberries and tried to PCR out our strawberry allergen (Fra a 1), but were unble ato do so # Looked to find introns ~ 800 bp to use to create our hairpins since it was difficult to acquire pHannibal vector w/ the pdk intron '''Future Directions''' # Look towards ordering [[Media:Aimrna.pdf|amiRNA]]for silencing # Waiting on arabidopsis plants to grow to extract genomic DNA (mon) # Look to find introns to use in creation of hairpin DNA # Cloning (digesting/ligating our sense/antisense/intron sequences necessary for hairpin creation into one vector to create biobricks) ==Team Fence== |} __NOTOC__

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