IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/18

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<p>To create the vector backbones we digested the E and R plasmids with HindIII and SpeI, and the O plasmids with SacII and SpeI. We ran the digested backbones on a gel and purified using the Qiaquick Gel Extraction kit. IMAGE HERE</p>
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Revision as of 15:24, 18 June 2010

iGEM iGarden Main project page
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Week 1 Summary (6/18/2010)

Team Vector

Image:PORE Vectors.xls

Aim: To biobrick agrobacteria vectors

We obtained the following pORE agrobacteria vectors from TAIR.

Name Original Name Description Plant Resistance
V1 pORE_O1 Agrobacterium vector open series pat
V2 pORE_O2 Agrobacterium vector open series nptll
V3 pORE_E3 Agrobacterium vector with ENTCUP2 promoter pat
V4 pORE_E4 Agrobacterium vector with ENTCUP2 promoter nptll
V5 pORE_R1 Agrobacterium vector with gusA reporter nptll
V6 pORE_R3 Agrobacterium vector with smgfp reporter nptll

To create the vector backbones we digested the E and R plasmids with HindIII and SpeI, and the O plasmids with SacII and SpeI. We ran the digested backbones on a gel and purified using the Qiaquick Gel Extraction kit. IMAGE HERE

Team Flavor

Team Allergy

Progress

  1. Identified allergens in arabidopsis, strawberries,tomatoes, and carrots and created primers for amplifying out these allergens (see primers and sequences)
  2. Extracted cDNA (rna extraction followed by rt-pcr) from strawberries and tried to PCR out our strawberry allergen (Fra a 1), but were unble ato do so
  3. Looked to find introns ~ 800 bp to use to create our hairpins since it was difficult to acquire pHannibal vector w/ the pdk intron

Future Directions

  1. Look towards ordering amiRNAfor silencing
  2. Waiting on arabidopsis plants to grow to extract genomic DNA (mon)
  3. Look to find introns to use in creation of hairpin DNA
  4. Cloning (digesting/ligating our sense/antisense/intron sequences necessary for hairpin creation into one vector to create biobricks)

Team Fence


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