IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/22: Difference between revisions
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*The above settings on the PCR machine were used | *The above settings on the PCR machine were used | ||
[[Image:cutout622.jpg|thumb|right]] | |||
To Make Agarose Gel | To Make Agarose Gel | ||
*150 mL TAE buffer combined with 1.5 g agarose in beaker | *150 mL TAE buffer combined with 1.5 g agarose in beaker |
Revision as of 11:42, 23 June 2010
iGEM iGarden | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ImagesTeam Vector: HindIII + PstI digest of miniprepped V9-V12 (#5,6) to check whether insert is present. Team FenceGAL4DBD Miniprep
PCRPCR program for LacIN modified after Monday's unsuccessful O2 and E10 PCR (no product) 1=95°C for 10:00 2=95°C for 00:15 3=50°C for 00:30 4=72°C for 01:30 5=steps 2-4 X 29 6=72°C for 10:00 7=4°C for ∞ PCR for E10 LacI
To Make Agarose Gel
DNA was extracted from gel by slicing out the bands and DNA was purified using the QIAquick Gel Extraction Kit, following protocol specific to using a centrifuge InnoculationsColonies from the following cultures were innoculated:
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