IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/22: Difference between revisions
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*final volume =90μL PCR product + 18μL 5X loading dye =110μL | *final volume =90μL PCR product + 18μL 5X loading dye =110μL | ||
DNA was extracted from gel by slicing out the bands and DNA was purified using the QIAquick Gel Extraction Kit, following protocol specific to using a centrifuge | DNA was extracted from gel by slicing out the bands and DNA was purified using the QIAquick Gel Extraction Kit, following protocol specific to using a centrifuge |
Revision as of 11:42, 23 June 2010
iGEM iGarden | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ImagesTeam Vector: HindIII + PstI digest of miniprepped V9-V12 (#5,6) to check whether insert is present. Team FenceGAL4DBD Miniprep
PCRPCR program for LacIN modified after Monday's unsuccessful O2 and E10 PCR (no product) 1=95°C for 10:00 2=95°C for 00:15 3=50°C for 00:30 4=72°C for 01:30 5=steps 2-4 X 29 6=72°C for 10:00 7=4°C for ∞ PCR for E10 LacI
To Make Agarose Gel
InnoculationsColonies from the following cultures were innoculated:
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