IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/25

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We miniprepped the cells to obtain the plasmids. We did so using the Qiaquick Miniprep kit. As an initial test that we had obtained the plasmid we expected we digested using EcoI and HindIII and ran on a gel. The gel (shown below) shows the presence of the expected bands.
We miniprepped the cells to obtain the plasmids. We did so using the Qiaquick Miniprep kit. As an initial test that we had obtained the plasmid we expected we digested using EcoI and HindIII and ran on a gel. The gel (shown below) shows the presence of the expected bands.
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To verify that we had inserted the sequence that we expected into the plasmid we planned on sending them for sequencing by GeneWiz. They required concentrations of 80ng/uL. Our nanodrop results showed that we had obtained low concentrations from our miniprep (<44ng/uL). To achieve higher concentrations of plasmid we re-picked colonies and incubated colonies for longer.
+
To verify that we had inserted the sequence that we expected into the plasmid we planned on sending them for sequencing by GeneWiz. GeneWiz require concentrations of 80ng/uL. Our nanodrop results showed that we had obtained low concentrations from our miniprep (<44ng/uL). To achieve higher concentrations of plasmid we re-picked colonies and incubated cultures for longer.
==Team Flavor==
==Team Flavor==

Revision as of 13:37, 25 June 2010

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Week 2 Summary (6/25/2010)

Team Vector

Last week we had made cultures of V9, V10, V11 and V12 vectors and stored pellets of the resulting cells over the weekend.

We miniprepped the cells to obtain the plasmids. We did so using the Qiaquick Miniprep kit. As an initial test that we had obtained the plasmid we expected we digested using EcoI and HindIII and ran on a gel. The gel (shown below) shows the presence of the expected bands.

To verify that we had inserted the sequence that we expected into the plasmid we planned on sending them for sequencing by GeneWiz. GeneWiz require concentrations of 80ng/uL. Our nanodrop results showed that we had obtained low concentrations from our miniprep (<44ng/uL). To achieve higher concentrations of plasmid we re-picked colonies and incubated cultures for longer.

Team Flavor

Team Allergy

Team Fence
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