IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/25
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Week 2 Summary (6/25/2010)Team VectorLast week we had made cultures of V9, V10, V11 and V12 vectors and stored pellets of the resulting cells over the weekend. We miniprepped the cells to obtain the plasmids. We did so using the Qiaquick Miniprep kit. As an initial test that we had obtained the plasmid we expected we digested using EcoI and HindIII and ran on a gel. The gel (shown below) shows the presence of the expected bands. To verify that we had inserted the sequence that we expected into the plasmid we planned on sending them for sequencing by GeneWiz. GeneWiz require concentrations of 80ng/uL. Our nanodrop results showed that we had obtained low concentrations from our miniprep (<44ng/uL). To achieve higher concentrations of plasmid we re-picked colonies and incubated cultures for longer. Team FlavorTeam AllergyTeam Fence |
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