IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/28: Difference between revisions
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==Team Flavour== | |||
===Miniprep of Miraculin and Brazzein Constructs=== | |||
Nanodrop Specs: | |||
{| class="wikitable" border ="1px solid black" | |||
|- | |||
!Name | |||
!Concentration (ng/μL) | |||
|- | |||
|Miraculin 1 | |||
|159.6 | |||
|- | |||
|Miraculin 2 | |||
|182.2 | |||
|- | |||
|Brazzein 1 | |||
|91.2 | |||
|- | |||
|Brazzein 2 | |||
|33.7 | |||
|- | |||
|} | |||
Miraculin 1: 159.6 ng/μL | |||
===DTL (Digestion, Ligation, Transformation) of The Big Three (pENTCUP2, NOSt, NOSt + STOP)=== | |||
Revision as of 11:41, 28 June 2010
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Entry title
PCR Reactions Mix of Parts 1,2,3 w/ primers A&B (simultaneous PCR) 3 reactions (GFP; Bet; LTP) Annealing Temp: 60C Extension Time: 15 sec
Team FlavourMiniprep of Miraculin and Brazzein ConstructsNanodrop Specs:
Miraculin 1: 159.6 ng/μL DTL (Digestion, Ligation, Transformation) of The Big Three (pENTCUP2, NOSt, NOSt + STOP)Mix of Parts 1&2 w/ Primers A&2 3 reactions (GFP; Bet; LTP) Mix of Parts 1&2 & 3 w/ Primers A&B Gels
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