IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/28: Difference between revisions
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===Miniprep of Miraculin and Brazzein Constructs=== | ===Miniprep of Miraculin and Brazzein Constructs=== | ||
*Protocol used was from the Qiagen Miniprep Kit | |||
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|+Nanodrop Specs | |+Nanodrop Specs |
Revision as of 11:51, 28 June 2010
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Entry title
PCR Reactions Mix of Parts 1,2,3 w/ primers A&B (simultaneous PCR) 3 reactions (GFP; Bet; LTP) Annealing Temp: 60C Extension Time: 15 sec
Team FlavourMiniprep of Miraculin and Brazzein Constructs
DTL (Digestion, Ligation, Transformation) of The Big Three (pENTCUP2, NOSt, NOSt + STOP)Mix of Parts 1&2 w/ Primers A&2 3 reactions (GFP; Bet; LTP) Mix of Parts 1&2 & 3 w/ Primers A&B Gels
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