IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/28: Difference between revisions
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===DTL (Digestion, Ligation, Transformation) of The Big Three (pENTCUP2, NOSt, NOSt + STOP)=== | ===DTL (Digestion, Ligation, Transformation) of The Big Three (pENTCUP2, NOSt, NOSt + STOP)=== | ||
{| border="1" | |||
|+ Digestion Reaction | |||
! !! pENTCUP2 !! NOSt !! NOSt+STOP !! B15 | |||
|- | |||
! DNA | |||
| 4 || 7 ||12 ||7 | |||
|- | |||
! NEB Buffer 3 (10x) | |||
| 2 || 2 || 2 || 2 | |||
| - | |||
! diH<sub>2</sub>O | |||
| 10 || 7 || 2 || 7 | |||
|- | |||
|} | |||
Revision as of 11:56, 28 June 2010
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Entry title
PCR Reactions Mix of Parts 1,2,3 w/ primers A&B (simultaneous PCR) 3 reactions (GFP; Bet; LTP) Annealing Temp: 60C Extension Time: 15 sec
Mix of Parts 1&2 w/ Primers A&2 3 reactions (GFP; Bet; LTP) Mix of Parts 1&2 & 3 w/ Primers A&B Gels Team FlavourMiniprep of Miraculin and Brazzein Constructs
DTL (Digestion, Ligation, Transformation) of The Big Three (pENTCUP2, NOSt, NOSt + STOP)
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