IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/28: Difference between revisions
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! NEB Buffer 3 (10x) | ! NEB Buffer 3 (10x) | ||
| 2 || 2 || 2 || 2 | | 2 || 2 || 2 || 2 | ||
| - | |- | ||
! diH<sub>2</sub>O | ! diH<sub>2</sub>O | ||
| 10 || 7 || 2 || 7 | | 10 || 7 || 2 || 7 | ||
|} | |} | ||
Revision as of 11:57, 28 June 2010
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Entry title
PCR Reactions Mix of Parts 1,2,3 w/ primers A&B (simultaneous PCR) 3 reactions (GFP; Bet; LTP) Annealing Temp: 60C Extension Time: 15 sec
Mix of Parts 1&2 w/ Primers A&2 3 reactions (GFP; Bet; LTP) Mix of Parts 1&2 & 3 w/ Primers A&B Gels Team FlavourMiniprep of Miraculin and Brazzein Constructs
DTL (Digestion, Ligation, Transformation) of The Big Three (pENTCUP2, NOSt, NOSt + STOP)
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