IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/29: Difference between revisions
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'''Digestions''' | '''Digestions''' | ||
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|+ Digestion Reactions | |+ ''Digestion Reactions'' | ||
! !! Bet!! GFP!! LTP!! LTP(.5)!! GFP(.5)!! Backbone (V0120 w/o a death gene) | ! !! Bet!! GFP!! LTP!! LTP(.5)!! GFP(.5)!! Backbone (V0120 w/o a death gene) | ||
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Revision as of 08:45, 29 June 2010
 iGEM iGarden
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Team AllergyDigestions
note: .5 denotes multistep pcr reaction from yesterday in which .5 uL of part 3 of gfp/ltp were use in one of the giant assemblies of parts 1,2,3 w/ primers A&B For LTP, LTP (.5), Bet For GFP For GFP (.5) Team FlavorResults of ligation and transformation of The Big Three (pENTCUP2, NosT, NosT + stop) with B15 backbone into E.coliA few colonies were found on the pENTCUP2 + Backbone plate, NosT + stop and backbone plate, and b15 backbone control plate. Colonies were picked from the plates and placed in 3ml of LB and agarose in a culture tube. The tubes were placed in the 37°C shaker. DLT of the Sweethearts - Miraculin and Brazzein (and B21)
Placed in 37°C waterbath for 20 minutes. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
