IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/30: Difference between revisions

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*Redid ligations and transformations (plated ~ 12 pm)
*Redid ligations and transformations (plated ~ 12 pm)
**Bet, LTP, GFP, LTP .5, GFP .5, Negative Control (just V0120 backbone)
**Bet, LTP, GFP, LTP .5, GFP .5, Negative Control (just V0120 backbone)
{| border="1"
|+ ''Ligation Reactions''
!  !! Bet!! GFP!! LTP!! LTP(.5)!! GFP(.5)!! Negative Control
|-
! DNA Insert
| align="center" | 1 || align="center" |  1|| align="center" | 1|| align="center" | 1|| align="center" | 1|| 0
|-
! T4 DNA ligase buffer (10x)
| align="center"  | 1 || align="center" |  1 || align="center" |  1|| align="center" |  1|| align="center" |  1|| align="center" |1 
|-
! diH<sub>2</sub>O
| align="center"  | 6 || align="center" |  6 || align="center" |  6|| align="center"  | 6|| align="center"  | 6|| align="center"| 7
|-
! T4 DNA ligase
| align="center"  | 1 || align="center" |  1 || align="center" |  1|| align="center" |  1|| align="center" |  1|| align = "center"| 1 
|-
! DNA Backbone
| align="center" | 1 || align="center" |  1|| align="center" | 1|| align="center" | 1|| align="center" | 1|| align="center" |1
|-
|}


==Team Flavor==
==Team Flavor==

Revision as of 09:09, 30 June 2010

iGEM iGarden <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Team Allergy

  • Checked for transformants (seems that only Bet worked but there are only 2 colonies (could be background))
    • Grew up 2 tubes for minipreps at ~10am (Should be ready ~ 4 pm)
  • Redid ligations and transformations (plated ~ 12 pm)
    • Bet, LTP, GFP, LTP .5, GFP .5, Negative Control (just V0120 backbone)
Ligation Reactions
Bet GFP LTP LTP(.5) GFP(.5) Negative Control
DNA Insert 1 1 1 1 1 0
T4 DNA ligase buffer (10x) 1 1 1 1 1 1
diH2O 6 6 6 6 6 7
T4 DNA ligase 1 1 1 1 1 1
DNA Backbone 1 1 1 1 1 1

Team Flavor

Miraculin, Brazzein

  • Colonies did grow!
  • Started 5 mL cultures of Miraculin and Brazzein biobrick constructs (5 cultures each)

The Big Three

  • Began miniprep of cultures of pENT, NOSt+STOP and our Control (for experimental purposes)



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