IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/01: Difference between revisions

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'''Sent amiRNA Bet for sequencing'''
'''Sent amiRNA Bet for sequencing'''
'''Digestion of V0120 (w/o death gene) for Ligation of antisense/sense sequences'''


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Revision as of 09:14, 1 July 2010

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Team Allergy

  • Ligations for full amiRNA and V0120 (w/o death gene)

note: .5 denotes multistep pcr reaction from yesterday in which .5 uL of part 3 of gfp/ltp were use in one of the giant assemblies of parts 1,2,3 w/ primers A&B

Ligation Reactions
Bet GFP LTP LTP(.5) GFP(.5)
DNA Insert (after 1:10 dilutions) 3 4.5 3 4.71 4.1
T4 DNA ligase buffer (10x) 2 2 2 2 2
diH2O 11.5 10 11.5 9.79 10.4
T4 DNA ligase 1 1 1 1 1
DNA Backbone 2.5 2.5 2.5 2.5 2.5
    • Used 50 ng of backbone and 150 ng of insert (ratio of insert to vector ~.14
    • Did 1:10 dilutions for the digested inserts

PCR of PME3 and PAL2

  • saw no bands from last night's pcr so we re ran it

Sent amiRNA Bet for sequencing

Digestion of V0120 (w/o death gene) for Ligation of antisense/sense sequences



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