IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/02: Difference between revisions
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* Check on sequences for Bet | * Check on sequences for Bet | ||
* Digest Pme3 and Pal 2 introns/Ligate w/ V0120/Transform | * Digest Pme3 and Pal 2 introns/Ligate w/ V0120/Transform | ||
* Check on | ** | ||
**Grow up colonies to miniprep, run on gel, sequence | * Check on transformations of amiRNA (5 transformations) and antisense/sense allergens (8 transformations) | ||
**There were colonies from the amiRNA transformations | |||
***We grew up 24 colonies (3 for each GFP, GFP .5, LTP, LTP .5, BET) ~ 10 am to be ready ~3 pm for minipreps) | |||
***The other 8 transformations did not show any colonies--we put it back in to see if we would get colonies (only 100 uL instead of 200 uL was plated) | |||
***To do: Grow up colonies to miniprep, run on gel, sequence | |||
==Team == | ==Team == | ||
Revision as of 07:21, 2 July 2010
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