IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/12: Difference between revisions

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#Redo Ligations for allergen Panel  
#Redo Ligations for allergen Panel  


==Results==
===Results===


'''Sequencing Results'''
'''Sequencing Results'''

Revision as of 11:27, 12 July 2010

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Team Allergy

Today, we received sequences for the allergen , intron, and amiRNA inserts. Since 4 of the 8 allergen inserts were not correctly done (and were not set for sequencing) and we want to repeat the inserts that DID work, we will redo ligations for the entire panel.

Procedures

  1. Check Sequencing Results
  2. Redo Ligations for allergen Panel

Results

Sequencing Results

    • all amiRNA sequences matched
    • Pal(#2) matched
    • LTP Sense and Ger Sense matched
    • Bet sense did not match

Redo Ligations w/ phosphatized backbone for entire allergen panel, PME, PAL, PDK

Add PDK to amiRNA vectors if sequences match up



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