IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/12: Difference between revisions
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**Bet sense did not match | **Bet sense did not match | ||
Redo Ligations w/ phosphatized backbone for entire allergen panel, PME, PAL | Redo Ligations w/ phosphatized backbone for entire allergen panel, | ||
PME did not grow, we will not use it since we have good PDK and PAL introns. | |||
PAL and PDK will be digested and inserted into a plasmid containing a sense strand of an allergen. | |||
Add PDK to amiRNA vectors if sequences match up | Add PDK to amiRNA vectors if sequences match up | ||
Revision as of 11:31, 12 July 2010
 iGEM iGarden
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Team AllergyToday, we received sequences for the allergen , intron, and amiRNA inserts. Since 4 of the 8 allergen inserts were not correctly done (and were not set for sequencing) and we want to repeat the inserts that DID work, we will redo ligations for the entire panel. Procedures
ResultsSequencing Results
Redo Ligations w/ phosphatized backbone for entire allergen panel, PME did not grow, we will not use it since we have good PDK and PAL introns. PAL and PDK will be digested and inserted into a plasmid containing a sense strand of an allergen. Add PDK to amiRNA vectors if sequences match up
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