IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/13: Difference between revisions
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{| border="1" | {| border="1" | ||
|+ ''Digestion Reactions'' | |+ ''Digestion Reactions for Backbones (plasmids containing sense)'' | ||
! !! pLTPS1!! pLTPS2!! pLTPS3!! GerS1 | ! !! pLTPS1!! pLTPS2!! pLTPS3!! GerS1 | ||
|- | |- | ||
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|- | |- | ||
! Pst | ! Pst | ||
| align="center" | 1 || align="center" | 1|| align="center" | 1|| align="center" | 1|| align="center" | 1 | | align="center" | 1 || align="center" | 1|| align="center" | 1|| align="center" | 1|| align="center" | | ||
|- | |||
|} | |||
{| border="1" | |||
|+ ''Digestion Reactions for Inserts'' | |||
! !! Pal2!! PDK | |||
|- | |||
! DNA (~ 1 ug) | |||
| align="center" |9|| align="center" | | |||
|- | |||
! FD Buffer (10x) | |||
| align="center" | 2 || align="center" |2 | |||
|- | |||
! diH<sub>2</sub>O | |||
| align="center" | 7 || align="center" | | |||
|- | |||
! Xba1 | |||
| align="center" | 1|| align="center" |1 | |||
|- | |||
! Pst1 | |||
| align="center" | 1|| align="center" |1 | |||
|- | |- | ||
|} | |} |
Revision as of 08:18, 13 July 2010
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Digestion
pLTPS1 | pLTPS2 | pLTPS3 | GerS1 | ||
---|---|---|---|---|---|
DNA | 10 | 8 | 7 | 10 | |
FD Buffer (10x) | 2 | 2 | 2 | 2 | |
diH2O | 6 | 8 | 9 | 6 | |
Spe | 1 | 1 | 1 | 1 | |
Pst | 1 | 1 | 1 | 1 |
Pal2 | PDK | |
---|---|---|
DNA (~ 1 ug) | 9 | |
FD Buffer (10x) | 2 | 2 |
diH2O | 7 | |
Xba1 | 1 | 1 |
Pst1 | 1 | 1 |