IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/15: Difference between revisions
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*Minipreps of PDK+V0120 and GerS+PDK (LTPS+ PDK did not grow) | *Minipreps of PDK+V0120 and GerS+PDK (LTPS+ PDK did not grow) | ||
**glycerol stocks 500uL of 80% glycerol + 500 uL cells | **glycerol stocks 500uL of 80% glycerol + 500 uL cells | ||
**Redoing Ligations/Transformations of LTPS+PDK | **Concentrations of GerS+PDK: 347.7 ng/uL; 428.6 ng/uL 541.6 ng/uL | ||
**Sending PDK+V0120 for sequencing | **Concentrations of PDK: 414.2 ng/uL; 478.8 ng/uL; 510.2 ng/uL; 119.2 ng/uL | ||
**''Waiting on LB+Amp plates'' | |||
***Redoing Ligations/Transformations of LTPS+PDK | |||
***Sending PDK+V0120 for sequencing | |||
*Looking at sequencing results for our other amiRNA parts | *Looking at sequencing results for our other amiRNA parts | ||
** | **Other minpreps of the artificial parts did not work | ||
** | **Repicking amiRNA colonies to miniprep | ||
*Minipreps of Bet 1S (2); Bet 1.2 S (1); Bet 1.2 A (1) | *Minipreps of Bet 1S (2); Bet 1.2 S (1); Bet 1.2 A (1) | ||
** | **Redoing Ligations & Transformations of Sense/Antisense Parts | ||
**Bet 1S: 161 ng/uL & 199.2 ng/uL; Bet 1.2 A: 199.4 ng/uL; Bet 1.2S: 162.5 ng/uL | |||
*Diagnostic Digest of (PDK+V0120; PDK+GERS; BetS; Bet 1.2S/A) | |||
*Looking at PCR results for Allergen Panel from Genomic DNA that we extracted (did not work) | *Looking at PCR results for Allergen Panel from Genomic DNA that we extracted (did not work) | ||
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[[Image: |240px|allergen panel from arabidopsis DNA extraction]] | [[Image: |240px|allergen panel from arabidopsis DNA extraction]] | ||
==Team Fence== | ==Team Fence== |
Revision as of 11:57, 15 July 2010
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Team Allergy
[[Image: |240px|allergen panel from arabidopsis DNA extraction]] Team FenceTeam FlavorMiraculin/Brazzein YFP N/C and NosT + Stop in V24 DUET vector
ODs Miraculin C1: 168.8 ng/μL Miraculin C2: 67.2 ng/μL Miraculin N1: 183.0 ng/μL Miraculin N2: 173.5 ng/μL Brazzein N1: 54.8 ng/μL Brazzein N2: 79.0 ng/μL Brazzein C1: 161.4 ng/μL Brazzein C2: 125.0 ng/μL
Expected Lengths Miraculin + YFP + NOSt + STOP = 700 + 1500 + 250 = 2450 bp Brazzein + YFP + NOSt + STOP = 300 + 1500 + 250 = 2050 bp V24 = 5200 bp Team Vector |