IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/16: Difference between revisions

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Diagnostic Gel: Ladder, Bet1-6, LTP1-LTP4, Ladder;  Ladder, LTP6, LTP.5 (1-6), Ladder  
Diagnostic Gel: Ladder, Bet1-6, LTP1-LTP4, Ladder;  Ladder, LTP6, LTP.5 (1-6), Ladder
 
[[Image:AmiRNA d digest.jpg|240px]]
==Team Fence==
==Team Fence==


Revision as of 14:24, 16 July 2010

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Team Allergy

More arabadopsis DNA arrived today. We are going to obtain more hpRNA parts and

  • Did a second PCR/Purification of our allergen panel from Julia's arabidopsis genomic DNA. It worked!

GFP didn't show up (first 2 lanes). The plants for the extraction weren't the GFP variety so this is expected.


  • gel purifying PCR products
    • Concentrations: LTPS: LTPA: GERS: GERA: BETS: BETA: BET2S: BET2A: PME: PAL:
  • Digested Xba1 Pst1/Ligated into V0120 (after phosphatizing backbone), transformed (LB amp plates)
  • Sent BetS, PDK2, and GerS (3) for sequencing
  • Miniprep amiRNA

Nanodrop of Miniprepped amiRNA Concentrations

Concentrations in ng/μL of colonies 1~6
1 2 3 4 5 6
LTP 320.7 359.4 290.2 238.5 N/A 212.4
Bet 156.8 267.7 340.0 287.4 278.9 273.5
LTP 0.5 296.4 268.8 244.2 212.0 292.6 208.3

Diagnostic Gel: Ladder, Bet1-6, LTP1-LTP4, Ladder; Ladder, LTP6, LTP.5 (1-6), Ladder

Team Fence


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