IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/19: Difference between revisions

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* Re-do of Valencene PCR (failed)
* Re-do of Valencene PCR (failed)
[[Image:Valencene_egel.jpg|240px]]


==Team Allergy==
==Team Allergy==

Revision as of 06:18, 20 July 2010

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Team Flavor

  • DTL of Mira/Brazz+StrepII into STOP+V0120
  • Confirm of valencene PCR (failed)

  • Re-do of Valencene PCR (failed)

Team Allergy

  • Got back sequencing results of GerS, BetS, PDK
    • All three samples of GerS sent in worked and PDK worked
  • Ran PCR of Friday's digested B21. The gel suggested a partial digest: we saw two bands of backbone where we should have seen only one:
  • Sending 6 samples of amiRNA (LTP.5), 5 samples of amiRNA (LTP), 6 samples of amiRNA (Bet)
  • Second digest of B21 appears to have been more successful:
  • Ligating and transforming backbone + allergen parts
  • Insert your content here.



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