IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/19: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 42: Line 42:
|-
|-
! DNA Insert
! DNA Insert
| align="center"  | 5 || align="center" |  20 || align="center" | 6 || align="center"  | 8 || align="center"  | 0
| align="center"  | 11 || align="center" |  11 || align="center" | 3 || align="center"  | 8 || align="center"  | 0
|-
|-
! DNA Backbone
! DNA Backbone
| align="center"  | 5 || align="center" |  5 || align="center" |  5 || align="center" |  5 || align="center" |  5
| align="center"  | 3 || align="center" |  3 || align="center" |  3 || align="center" |  3 || align="center" |  3
|-
|-
! T4 DNA ligase  
! T4 DNA ligase  
Line 51: Line 51:
|-
|-
|}
|}


===Confirm of valencene PCR (failed)===
===Confirm of valencene PCR (failed)===

Revision as of 07:28, 20 July 2010

iGEM iGarden <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Team Flavor

DTL of Mira/Brazz+StrepII into STOP+V0120

Digestion

Digestion Reactions
Stop v0120 1 Stop v0120 2
DNA 10 8
FD Buffer (10x) 2 2
diH2O 6 8
Xba1 1 1
EcoRI 1 1
Ligation Reactions
Mira N + Stop Mira C + Stop Brazz N + Stop Brazz C + Stop Stop only Control
diH2O 3 3 11 6 14
T4 DNA ligase buffer (10x) 2 3 2 2 2
DNA Insert 11 11 3 8 0
DNA Backbone 3 3 3 3 3
T4 DNA ligase 1 1 1 1 1


Confirm of valencene PCR (failed)

  • Re-do of Valencene PCR (failed)

Team Allergy

  • Got back sequencing results of GerS, BetS, PDK
    • All three samples of GerS sent in worked and PDK worked
  • Ran PCR of Friday's digested B21. The gel suggested a partial digest: we saw two bands of backbone where we should have seen only one:
  • Sending 6 samples of amiRNA (LTP.5), 5 samples of amiRNA (LTP), 6 samples of amiRNA (Bet)
  • Second digest of B21 appears to have been more successful:
  • Ligating and transforming backbone + allergen parts
  • Insert your content here.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010/2010/07/19&oldid=434285"