IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/21
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Team AllergyConcentrations of Allergen Parts and Introns:
Diagnostic Digest lader; ladder; GerS (1-3); Ger A (1-3); Bet2S (1-3); Bet2A (1-3); BetA (!-3); LTPS (1-3); LTPA (1-3); Pal (1-3); ladder; ladder (More contrasted image below):
Team FenceNanodroping 5xGal promoterNanodrop of ligated annealed oligos: 5200.8 ng/μL, 1.32 260/280 E-gel of 5xGal promoterRan 2% E-gel of .5μL of the ligation reaction (2.6μg) in 15μL water, with 10μL of KB plus ladder on either side. Ladder ran screwy, so new ladder used,and a 2% agarose Gel was poured for gel extraction Gel Extraction of 5xGal promoterpoured 2% agarose Gel for gel extraction, ran 2μL of the ligated annealing reaction in 13μL EB buffer, with two KB plus 1 ladders, one on each side.
Team FlavorLigation/Transformation of Mira/Brazz+StrepII
NEB T4 Ligase was used for all reactions Digestion & Gel Purification of V24
1039.9 ng of DNA was digested in both reactions Digestion reactions were run on a 1% Agarose gel at 125V for 30 min Specs V24-1 N/S: 13.5 ng/μL V24-1 N/S: 17.9 ng/μL
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