IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/26: Difference between revisions
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10μL DNTP | 10μL DNTP | ||
==Team Allergy== | |||
*minipreps of v0120sense+pdk/pal intron parts | |||
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Revision as of 09:30, 26 July 2010
 iGEM iGarden
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Team FencePFX polymerase PCR 1μL 10mM MgSO4 2μL 10X pfx amplification buffer 2μL 10X PCRx enhancer solution 1μL F primer 1μL R primer 0.5μL pfx polymerase 2μL DNTP 9.5μL total DNA: 21ng/μL 50 to 200μL DNA per reaction 5 reactions 4μL per reaction = 20μL sample total (MM) 9.5μL + 4μL = 13.5μL therefore 6.5μL H20 per reaction MM 5μL 10nM MgSO4 10μL 10X pfx amplification buffer 10μL 10X PCRx enhancer solution 5μL F primer 5μL R primer 2.5μL pfx polymerase 10μL DNTP Team Allergy
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