IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/26: Difference between revisions
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*minipreps of v0120sense+pdk/pal intron parts | *minipreps of v0120sense+pdk/pal intron parts | ||
*Digest vectors w/ Spe and Pst1 and ligate with antisense parts (already digested and gel purified) | *Digest vectors w/ Spe and Pst1 and ligate with antisense parts (already digested and gel purified) | ||
==Team Flavor== | |||
*Digested miniprepped Mira/Brazz+StrepII+STOP constructs with Not1/Spe1 for ligation confirmation: failed. | |||
*Ran PCR product of Valencia Orange genome to confirm: failed. | |||
[[Image:StrepStopNotSpeValPCR.jpg|240px]] | |||
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Revision as of 11:11, 26 July 2010
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Team FencePFX polymerase PCR
9.5μL total DNA: 21ng/μL
9.5μL + 4μL = 13.5μL therefore 6.5μL H20 per reaction MM
total volume:100μL Team Allergy
Team Flavor
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