IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/27: Difference between revisions

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Image: Ladder (every 6)
Image: Ladder (every 6)


[[Image: |240px]]
[[Image: Ddigestrun2.jpg|240px]]
 
[[Image: Ddigestrun3.jpg|240px]]
[[Image: Ddigestrun3.jpg|240px]]


Revision as of 08:51, 27 July 2010

iGEM iGarden <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Team Allergy

  • Diagnostic Digest (EcoR1 & Pst1)

1-6: LTP + PDK (3200 & 1200);

7-16: LTP + PAL (3200 & 540);

17,18: Bet1 + PDK (3200 & 1200);

19-22: Bet1 + PAL (3200 & 540);

23,24: Bet2+PDK (3200 & 1200);

25-28: Bet2 + PAL (3200 & 540);

29-32: Ger3 + PDK (3200 & 1200);

33-38: Ger3 + PAL (3200 & 540);

Image: Ladder (every 6)


  • Two LTPS+Pal worked (9,11), 3 Ger3S+Pal worked (36-38), 4 Bet2S+Pal Worked (25-28)
    • Digested these parts with spe and pst1 (slow digest)

Antisense-Intron Backbone Digestion

1-39
DNA ~1.5 ug
10x BSA 3
diH2O Fill to 30
Spe 1.5
Pst1 1.5
Neb Buffer 2 (10x) 3

(Let it go for 2 hours and then phosphatized backbone (20 min)

  • Ligated with antisense parts and transformed



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