IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/27

Team Allergy

• Diagnostic Digest (EcoR1 & Pst1)

1-6: LTP + PDK (3200 & 1200);

7-16: LTP + PAL (3200 & 540);

17,18: Bet1 + PDK (3200 & 1200);

19-22: Bet1 + PAL (3200 & 540);

23,24: Bet2+PDK (3200 & 1200);

25-28: Bet2 + PAL (3200 & 540);

29-32: Ger3 + PDK (3200 & 1200);

33-38: Ger3 + PAL (3200 & 540);

Image: Ladder (every 6)

• Two LTPS+Pal worked (9,11), 3 Ger3S+Pal worked (36-38), 4 Bet2S+Pal Worked (25-28)
  • Digested these parts with spe and pst1 (slow digest)

Antisense-Intron Backbone Digestion

(Let it go for 2 hours and then phosphatized backbone (20 min)

• Ligated with antisense parts and transformed