IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/27
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Team Allergy
1-6: LTP + PDK (3200 & 1200); 7-16: LTP + PAL (3200 & 540); 17,18: Bet1 + PDK (3200 & 1200); 19-22: Bet1 + PAL (3200 & 540); 23,24: Bet2+PDK (3200 & 1200); 25-28: Bet2 + PAL (3200 & 540); 29-32: Ger3 + PDK (3200 & 1200); 33-38: Ger3 + PAL (3200 & 540); Image: Ladder (every 6)
Antisense-Intron Backbone Digestion
(Let it go for 2 hours and then phosphatized backbone (20 min)
Team FlavorLigation of Mira/Brazz + StrepII to STOP + V0120
Miraculin: 34ng Brazzein: 11ng Backbone: 50ng
PCR of Valencene using Pfx Polymerase
Specs: DNA 1-1: 1.8 ng/μL DNA 1-2: 1.1 ng/μL DNA 2-1: 2.6 ng/μL DNA 2-2: 2.2 ng/μL
Mira/Brazz+StrepII+STOP confirmation digestDigested DNA: MN2: 211.2 ng MC2: 190.2 ng BN2: 184.4 ng BC2: 219.2 ng
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