IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/29

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(Team Allergy)
(Team Allergy)
Line 8: Line 8:
* Rules the world.
* Rules the world.
* Also, we ran a gel for our second attempt at amiRNA stiching (stage 1): [[Image:AmiRNA_stich2_stage1.jpg|50px]]. It seems that the backbone is still present at high concentrations after PCR purification. Maybe this is why last time we had a high incidence of background? This time we're gel purifying.
* Also, we ran a gel for our second attempt at amiRNA stiching (stage 1): [[Image:AmiRNA_stich2_stage1.jpg|50px]]. It seems that the backbone is still present at high concentrations after PCR purification. Maybe this is why last time we had a high incidence of background? This time we're gel purifying.
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 +
==Team Flavor==
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===Minipreps===
 +
*Miniprep of Mira/Brazz+StrepII+STOP in V0120
 +
===Gel Extraction===
 +
*Gel extraction of J45017: <font color="orange"> yellow </font>

Revision as of 11:11, 29 July 2010

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Team Allergy

  • Rules the world.
  • Also, we ran a gel for our second attempt at amiRNA stiching (stage 1): . It seems that the backbone is still present at high concentrations after PCR purification. Maybe this is why last time we had a high incidence of background? This time we're gel purifying.

Team Flavor

Minipreps

  • Miniprep of Mira/Brazz+StrepII+STOP in V0120

Gel Extraction

  • Gel extraction of J45017: yellow



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