IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/29
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==Team Allergy== | ==Team Allergy== | ||
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| - | * | + | * Ran a gel for our second attempt at amiRNA stiching (stage 1): [[Image:AmiRNA_stich2_stage1.jpg|50px]]. It seems that the backbone is still present at high concentrations after PCR purification. Maybe this is why last time we had a high incidence of background? This time we're gel purifying. |
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| + | *Dignostic Digest | ||
| + | '''Gel:''' | ||
| + | ladder, 9, 9, 11, 11, 25, 25, 28, 28, ladder, 36, 36, 37, 37, 38, 38, ladder, LTPS+ PDK, LTPS+PDK, Bet2S+PDK, Bet2S+PDK, GerS+PDK, GerS+PDK, GerS+PDK, GerS+PDK | ||
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| + | '''Expected band lengths:''' | ||
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| + | ''Complete Parts'' | ||
| + | 9,11 (LTPS+Pal+LTPA): 840bp and 3200 bp | ||
| + | 25,28 (Bet2S+Pal+Bet2A): 840bp and 3200 bp | ||
| + | 36,37,38 (GerS+Pal+GerA): 840bp and 3200 bp | ||
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| + | ''Sense + Intron Parts'' | ||
| + | LTPS+ PDK: 1200 and 3200 bp | ||
| + | Bet2S+ PDK: 1200 and 3200 bp | ||
| + | GerS+PDK: 1200 and 3200 bp | ||
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| + | [[Image: |240 px]] | ||
==Team Flavor== | ==Team Flavor== | ||
Revision as of 11:57, 29 July 2010
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Team Allergy
Gel: ladder, 9, 9, 11, 11, 25, 25, 28, 28, ladder, 36, 36, 37, 37, 38, 38, ladder, LTPS+ PDK, LTPS+PDK, Bet2S+PDK, Bet2S+PDK, GerS+PDK, GerS+PDK, GerS+PDK, GerS+PDK Expected band lengths: Complete Parts 9,11 (LTPS+Pal+LTPA): 840bp and 3200 bp 25,28 (Bet2S+Pal+Bet2A): 840bp and 3200 bp 36,37,38 (GerS+Pal+GerA): 840bp and 3200 bp Sense + Intron Parts LTPS+ PDK: 1200 and 3200 bp Bet2S+ PDK: 1200 and 3200 bp GerS+PDK: 1200 and 3200 bp [[Image: |240 px]] Team FlavorMinipreps
Gel Extraction
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