IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/29

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(Gel Extraction)
(Team Allergy)
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==Team Allergy==
==Team Allergy==
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* Rules the world.
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* Also, we ran a gel for our second attempt at amiRNA stiching (stage 1): [[Image:AmiRNA_stich2_stage1.jpg|50px]]. It seems that the backbone is still present at high concentrations after PCR purification. Maybe this is why last time we had a high incidence of background? This time we're gel purifying.
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* Ran a gel for our second attempt at amiRNA stiching (stage 1): [[Image:AmiRNA_stich2_stage1.jpg|50px]]. It seems that the backbone is still present at high concentrations after PCR purification. Maybe this is why last time we had a high incidence of background? This time we're gel purifying.
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*Dignostic Digest
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'''Gel:'''
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ladder, 9, 9, 11, 11, 25, 25, 28, 28, ladder, 36, 36, 37, 37, 38, 38, ladder, LTPS+ PDK, LTPS+PDK, Bet2S+PDK, Bet2S+PDK, GerS+PDK, GerS+PDK, GerS+PDK, GerS+PDK
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'''Expected band lengths:'''
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''Complete Parts''
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9,11 (LTPS+Pal+LTPA): 840bp and 3200 bp
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25,28 (Bet2S+Pal+Bet2A): 840bp and 3200 bp
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36,37,38 (GerS+Pal+GerA): 840bp and 3200 bp
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''Sense + Intron Parts''
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LTPS+ PDK: 1200 and 3200 bp
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Bet2S+ PDK: 1200 and 3200 bp
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GerS+PDK: 1200 and 3200 bp
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[[Image: |240 px]]
==Team Flavor==
==Team Flavor==

Revision as of 11:57, 29 July 2010

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Team Allergy

  • Ran a gel for our second attempt at amiRNA stiching (stage 1): . It seems that the backbone is still present at high concentrations after PCR purification. Maybe this is why last time we had a high incidence of background? This time we're gel purifying.
  • Dignostic Digest

Gel: ladder, 9, 9, 11, 11, 25, 25, 28, 28, ladder, 36, 36, 37, 37, 38, 38, ladder, LTPS+ PDK, LTPS+PDK, Bet2S+PDK, Bet2S+PDK, GerS+PDK, GerS+PDK, GerS+PDK, GerS+PDK

Expected band lengths:

Complete Parts 9,11 (LTPS+Pal+LTPA): 840bp and 3200 bp 25,28 (Bet2S+Pal+Bet2A): 840bp and 3200 bp 36,37,38 (GerS+Pal+GerA): 840bp and 3200 bp

Sense + Intron Parts LTPS+ PDK: 1200 and 3200 bp Bet2S+ PDK: 1200 and 3200 bp GerS+PDK: 1200 and 3200 bp

[[Image: |240 px]]

Team Flavor

Minipreps

  • Miniprep of Mira/Brazz+StrepII+STOP in V0120

Gel Extraction

  • Gel extraction of J45017: upcoming



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